| Title |
Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver
|
|---|---|
| Published in |
Glycoconjugate Journal, June 1994
|
| DOI | 10.1007/bf00731218 |
| Pubmed ID | |
| Authors |
Petra Schneckenburger, Lee Shaw, Roland Schauer |
| Abstract |
CMP-N-acetylneuraminate hydroxylase was isolated from mouse liver high speed supernatant with a yield of 0.4% and an apparent 1000-fold purification. The enzyme is a monomeric protein with a molecular weight of 66 kDa, as determined by gel filtration and SDS-PAGE. The hydroxylase system was reconstituted with Triton X-100-solubilized mouse liver microsomes and purified soluble or microsomal forms of cytochrome b5 reductase and cytochrome b5. The systems were characterized in detail and kinetic parameters for each system were determined. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 100% |
| Researcher | 1 | 100% |
| Other | 1 | 100% |
| Student > Master | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 100% |
| Agricultural and Biological Sciences | 1 | 100% |
| Psychology | 1 | 100% |
| Chemistry | 1 | 100% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 December 2015.
All research outputs
#5,447,195
of 25,374,917 outputs
Outputs from Glycoconjugate Journal
#114
of 929 outputs
Outputs of similar age
#2,748
of 20,889 outputs
Outputs of similar age from Glycoconjugate Journal
#2
of 3 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 929 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 20,889 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.