| Title |
Genetic contexts related to the diffusion of plasmid-mediated CTX-M-55 extended-spectrum beta-lactamase isolated from Enterobacteriaceae in China
|
|---|---|
| Published in |
Annals of Clinical Microbiology and Antimicrobials, March 2018
|
| DOI | 10.1186/s12941-018-0265-x |
| Pubmed ID | |
| Authors |
Xiaoxin Hu, Jianjun Gou, Xiaobing Guo, Zaiqiu Cao, Yuan Li, Hongjian Jiao, Xiaohong He, Yihui Ren, Fuyun Tian |
| Abstract |
CTX-M-55 extended-spectrum beta-lactamases are being rapidly disseminated and transmitted in clinical practices around the world. The genetic contexts of the transferable plasmid-mediated blaCTX-M-55gene in Enterobacteriaceae were detected and characterized in this study. Isolates were obtained from the First Affiliated Hospital of Zhengzhou University between September 2015 and March 2016. Based on polymerase chain reaction and BLAST analysis, resistance genes and genetic context of the blaCTX-M-55gene were investigated. Conjugation experiments and multilocus sequence typing were performed to demonstrate plasmid-mediated blaCTX-M-55transmission. Thirteen blaCTX-M-55-positive isolates of Enterobacteriaceae were obtained. Seven isolates were Escherichia coli, 3 were Klebsiella pneumoniae, 1 was Citrobacter freundii, 1 was Morganella morganii and 1 was Serratia marcescens. The blaCTX-M-55gene has not previously been identified from C. freundii and M. morganii. Four different blaCTX-M-55genetic contexts were identified, and all of them harbored ISEcp1 in the region upstream of blaCTX-M-55(in two cases, ISEcp1 was truncated by IS26, and in one case, it was truncated by IS1294), whereas ORF477 was detected downstream of the blaCTX-M-55gene from 12 of 13 strains. The novel genetic context of ISEcp1∆-blaCTX-M-55-∆IS903 was firstly detected the IS903 element which was identified downstream of blaCTX-M-55. A conjugation assay revealed that all blaCTX-M-55plasmids were quickly and easily transferable to recipient E. coli, which then presented resistance to multiple antibiotics. Numerous blaCTX-M-55-positive strains were isolated in a short period of 7 months. The findings indicate that blaCTX-M-55was rapidly disseminated. The genetic context and conjugative transfer found in this study demonstrate that there is active transmission of blaCTX-M-55among strains of Enterobacteriaceae in China, which could give rise to an urgent global public health threat. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 21% |
| Student > Master | 4 | 17% |
| Student > Ph. D. Student | 3 | 13% |
| Lecturer | 2 | 8% |
| Student > Doctoral Student | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 7 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 5 | 21% |
| Veterinary Science and Veterinary Medicine | 4 | 17% |
| Medicine and Dentistry | 3 | 13% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Computer Science | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 7 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2018.
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#20,469,520
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Outputs from Annals of Clinical Microbiology and Antimicrobials
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Outputs of similar age from Annals of Clinical Microbiology and Antimicrobials
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