| Title |
Involvement of RFX proteins in transcriptional activation from a Ras-responsive enhancer element
|
|---|---|
| Published in |
Archives of Dermatological Research, March 2004
|
| DOI | 10.1007/s00403-004-0456-5 |
| Pubmed ID | |
| Authors |
Susann Maijgren, Inderpreet Sur, Mats Nilsson, Rune Toftgård |
| Abstract |
The B10.RRE element has previously been shown to mediate induced transcription in response to an activated Ha- ras gene and epidermal growth factor in keratinocytes but not in fibroblasts. We report the identification of regulatory factor for X box 3 (RFX-3) as a B10.RRE-binding protein, using the yeast one-hybrid assay. We showed that in vitro-translated RFX3, as well as RFX1 and RFX2, was able to bind B10.RRE in a sequence-specific manner. Furthermore, RFX proteins are part of the protein complex in HeLa and HepG2 cells that binds the B10.RRE element. Functional analysis in cell culture demonstrated that a truncated version of RFX1 (*RFX1) lacking the repressor domain was able to activate transcription from B10.RRE. Conversely, a dominant-negative form of RFX1 was able to block Ras-induced transcription. Taken together, these results suggest a novel role for the RFX family of transcription factors as modulators of Ras signalling in epithelial cells. Such an interaction is of potential relevance for cell growth and carcinogenesis in the skin. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 13% |
| Unknown | 7 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 50% |
| Student > Ph. D. Student | 3 | 38% |
| Professor | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 63% |
| Biochemistry, Genetics and Molecular Biology | 2 | 25% |
| Medicine and Dentistry | 1 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#8,534,976
of 25,373,627 outputs
Outputs from Archives of Dermatological Research
#384
of 1,445 outputs
Outputs of similar age
#22,434
of 63,372 outputs
Outputs of similar age from Archives of Dermatological Research
#4
of 7 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,445 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
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