| Title |
Characterization and expression of three novel differentiation-related genes belong to the human NDRG gene family
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, January 2002
|
| DOI | 10.1023/a:1017934810825 |
| Pubmed ID | |
| Authors |
Xianghu Qu, Yun Zhai, Handong Wei, Chenggang Zhang, Guichun Xing, Yongtao Yu, Fuchu He |
| Abstract |
NDRG1 (N-Myc downstream regulated) is upregulated during cell differentiation, repressed by N-myc and c-myc in embryonic cells, and suppressed in several tumor cells. A nonsense mutation in the NDRG1 gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom (HMSNL), indicating that NDRG1 functions in the peripheral nervous system necessary for axonal survival. Here, we cloned three human cDNAs encoding NDRG2 (371aa), NDRG3 (375aa) and NDRG4 (339aa), which are homologous to NDRG1. These three genes, together with NDRG1, constitute the NDRG gene family. The phylogenetic analysis of the family demonstrated that human NDRG1 and NDRG3 belong to a subfamily, and NDRG2 and NDRG4 to another. At amino acid (aa) level, the four members share 53-65% identity. Each of the four proteins contains an alpha/beta hydrolase fold as in human lysosomal acid lipase. Expression of the fusion proteins NDRG2/GFP, NDRG3/GFP and NDRG4/GFP in COS-7 cells showed that all of them are cytosolic proteins. Based on UniGene cluster analysis, the genes NDRG2, NDRG3 and NDRG4 are located at chromosome 14q11.1-11.2, 20q12-11.23 and 16q21-22.1, respectively. Northern and dot blot analysis shows that all of the three genes are highly expressed in adult brain and almost not detected in the eight human cancer lines. In addition, in contrast to the relatively ubiquitous expression of NDRG1, NDRG2 is highly expressed in adult skeletal muscle and brain, NDRG3 highly expressed in brain and testis, and NDRG4 specifically expressed in brain and heart, suggesting that they might display different specific functions in distinct tissues. |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 2% |
| Unknown | 45 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 22% |
| Student > Ph. D. Student | 10 | 22% |
| Student > Master | 5 | 11% |
| Student > Bachelor | 3 | 7% |
| Student > Postgraduate | 3 | 7% |
| Other | 4 | 9% |
| Unknown | 11 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 24% |
| Biochemistry, Genetics and Molecular Biology | 8 | 17% |
| Medicine and Dentistry | 6 | 13% |
| Neuroscience | 4 | 9% |
| Immunology and Microbiology | 1 | 2% |
| Other | 3 | 7% |
| Unknown | 13 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 September 2023.
All research outputs
#8,534,976
of 25,374,647 outputs
Outputs from Molecular and Cellular Biochemistry
#481
of 2,447 outputs
Outputs of similar age
#32,444
of 130,780 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#4
of 10 outputs
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So far Altmetric has tracked 2,447 research outputs from this source. They receive a mean Attention Score of 4.2. This one has gotten more attention than average, scoring higher than 51% of its peers.
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