| Title |
Human pituitary tumours express the bHLH transcription factors NeuroD1 and ASH1
|
|---|---|
| Published in |
Journal of Endocrinological Investigation, April 2014
|
| DOI | 10.1007/bf03348192 |
| Pubmed ID | |
| Authors |
E. Ferretti, D. Di Stefano, F. Zazzeroni, R. Gallo, A. Fratticci, R. Carfagnini, S. Angiulli, A. Santoro, G. Minniti, G. Tamburrano, E. Alesse, G. Cantore, A. Gulino, M. L. Jaffrain-Rea |
| Abstract |
Among the transcription factors involved in pituitary ontogenesis and physiology, basic helix-loop-helix (bHLH) have been poorly studied. Members of bHLH family include NeuroD1 and ASH1, both involved in neuroendocrine differentiation. We evaluated their mRNA expression patterns, by semi-quantitative RT-PCR analysis (sq-RT-PCR) and/or Northern blot, in a series of 33 pituitary adenomas (PA), anterior pituitaries, and pituitary cell lines. Immunohistochemistry for NeuroD1 was also performed in 25 PA. Low levels of NeuroD1 were observed in normal pituitaries and in the somatomammotroph cell lines GH3/GH4C1, contrasting with high levels in corticotroph AtT20 cells. NeuroD1 mRNA was widely expressed in PA (82%), with measurable levels found especially in those derived from Pit-1 independent lineages, i.e. corticotroph (5/5) and clinically non-secreting (CNS) adenomas (9/11). According to sq-RT-PCR analysis, overexpression of NeuroD1 compared to normal pituitaries was frequent. Variable nuclear NeuroD1 immunopositivity was also present in about 70% of studied cases. ASH1 mRNA was widely detected in normal pituitaries, in all tumour cell lines and in most PA (84%), with measurable levels in corticotroph (5/5) and CNS (9/11) adenomas, and in a significant subset of PA derived from Pit-1 dependent lineages (9/16). We conclude that: a) NeuroD1 is differentially expressed in PA and its possible ontogenetic and/or pathogenetic implications in non-corticotroph PA are discussed; b) ASH1 is a neuroendocrine marker whose expression is largely conserved in normal and neoplastic pituitary cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 27% |
| Professor > Associate Professor | 3 | 20% |
| Professor | 1 | 7% |
| Student > Doctoral Student | 1 | 7% |
| Researcher | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 4 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 27% |
| Medicine and Dentistry | 2 | 13% |
| Agricultural and Biological Sciences | 1 | 7% |
| Nursing and Health Professions | 1 | 7% |
| Neuroscience | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 5 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2007.
All research outputs
#8,517,130
of 25,392,205 outputs
Outputs from Journal of Endocrinological Investigation
#432
of 1,619 outputs
Outputs of similar age
#79,371
of 237,683 outputs
Outputs of similar age from Journal of Endocrinological Investigation
#93
of 467 outputs
Altmetric has tracked 25,392,205 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,619 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.4. This one has gotten more attention than average, scoring higher than 51% of its peers.
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We're also able to compare this research output to 467 others from the same source and published within six weeks on either side of this one. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.