| Title |
Characterization and Analysis of Posttranslational Modifications of the Human Large Cytoplasmic Ribosomal Subunit Proteins by Mass Spectrometry and Edman Sequencing
|
|---|---|
| Published in |
The Protein Journal, April 2003
|
| DOI | 10.1023/a:1025068419698 |
| Pubmed ID | |
| Authors |
Tatyana I. Odintsova, Eva-Christina Müller, Anton V. Ivanov, Tsezi A. Egorov, Ralf Bienert, Serguei N. Vladimirov, Susanne Kostka, Albrecht Otto, Brigitte Wittmann-Liebold, Galina G. Karpova |
| Abstract |
The 60S ribosomal proteins were isolated from ribosomes of human placenta and separated by reversed phase HPLC. The fractions obtained were subjected to trypsin and Glu-C digestion and analyzed by mass fingerprinting (MALDI-TOF), MS/MS (ESI), and Edman sequencing. Forty-six large subunit proteins were found, 22 of which showed masses in accordance with the SwissProt database (June 2002) masses (proteins L6, L7, L9, L13, L15, L17, L18, L21, L22, L24, L26, L27, L30, L32, L34, L35, L36, L37, L37A, L38, L39, L41). Eleven (proteins L7, L10A, L11, L12, L13A, L23, L23A, L27A, L28, L29, and P0) resulted in mass changes that are consistent with N-terminal loss of methionine, acetylation, internal methylation, or hydroxylation. A loss of methionine without acetylation was found for protein L8 and L17. For nine proteins (L3, L4, L5, L7A, L10, L14, L19, L31, and L40), the molecular masses could not be determined. Proteins P1 and protein L3-like were not identified by the methods applied. |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Norway | 1 | 2% |
| Unknown | 63 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 23% |
| Researcher | 13 | 20% |
| Student > Master | 9 | 14% |
| Professor > Associate Professor | 3 | 5% |
| Student > Doctoral Student | 3 | 5% |
| Other | 10 | 16% |
| Unknown | 11 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 28 | 44% |
| Biochemistry, Genetics and Molecular Biology | 9 | 14% |
| Neuroscience | 4 | 6% |
| Chemistry | 2 | 3% |
| Medicine and Dentistry | 2 | 3% |
| Other | 7 | 11% |
| Unknown | 12 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 December 2022.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from The Protein Journal
#163
of 639 outputs
Outputs of similar age
#20,903
of 63,302 outputs
Outputs of similar age from The Protein Journal
#2
of 4 outputs
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