| Title |
DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6
|
|---|---|
| Published in |
Plant Cell Reports, March 2018
|
| DOI | 10.1007/s00299-018-2282-9 |
| Pubmed ID | |
| Authors |
Yongqiang Zhang, Zhongjuan Liu, Xiaoyun Wang, Jianfeng Wang, Kai Fan, Zhaowei Li, Wenxiong Lin |
| Abstract |
DELLA proteins' negative regulation of dark-induced senescence and chlorophyll degradation in Arabidopsis is through interaction with WRKY6 and thus repression of its transcriptional activities on senescence-related genes. Senescence is an intricate and highly orchestrated process regulated by numerous endogenous and environmental signals. Gibberellins (GAs) and their signaling components DELLA proteins have been known to participate in the regulation of senescence. However, the mechanism of the GA-DELLA system involved in the senescence process remains largely unclear. Darkness is a known environmental factor that induces plant senescence. In this study, exogenous GA3(an active form of GA) accelerated but paclobutrazol (a specific GA biosynthesis inhibitor) retarded dark-induced leaf yellowing in Arabidopsis. Moreover, the dark-triggered decrease in chlorophyll content, increase in cell membrane leakage, and upregulation of senescence-associated genes were notably impaired in both endogenous GA-decreased mutants ga3ox1/ga3ox2 and ga20ox1/ga20ox2 compared with those in wild-type Col-0. These effects of darkness were enhanced in the quintuple mutant of DELLA genes gai-t6/rga-t2/rgl1-1/rgl2-1/rgl3-1 and conversely attenuated in the gain-of-function mutant gai and transgenic plant 35S::TAP-RGAd17 compared with wild-type Ler. Subsequently, RGA interacted with the transcription factor WRKY6 in a yeast two-hybrid assay, as confirmed by bimolecular fluorescence complementation and pull-down analyses. In addition, mutation and overexpression of WRKY6 retarded and accelerated dark-induced senescence, respectively. Furthermore, transient expression assays in Arabidopsis protoplasts indicated that RGA and GAI weakened the transcriptional activities of WRKY6 on its downstream senescence-related genes, including SAG13 and SGR. Taken together, these results suggest that GAs positively and DELLAs negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis. DELLAs function in this process, at least in part, by interacting with WRKY6. |
X Demographics
The data shown below were collected from the profiles of 20 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 3 | 15% |
| United Kingdom | 2 | 10% |
| Germany | 2 | 10% |
| Korea, Republic of | 1 | 5% |
| Portugal | 1 | 5% |
| Mexico | 1 | 5% |
| Canada | 1 | 5% |
| Ireland | 1 | 5% |
| Unknown | 8 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 50% |
| Scientists | 10 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 60 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 20% |
| Student > Master | 6 | 10% |
| Student > Ph. D. Student | 5 | 8% |
| Student > Bachelor | 4 | 7% |
| Professor | 4 | 7% |
| Other | 12 | 20% |
| Unknown | 17 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 27 | 45% |
| Biochemistry, Genetics and Molecular Biology | 13 | 22% |
| Medicine and Dentistry | 2 | 3% |
| Unknown | 18 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 November 2020.
All research outputs
#3,035,956
of 24,834,604 outputs
Outputs from Plant Cell Reports
#112
of 2,323 outputs
Outputs of similar age
#60,856
of 336,614 outputs
Outputs of similar age from Plant Cell Reports
#5
of 26 outputs
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