| Title |
HSP70-2 is part of the synaptonemal complex in mouse and hamster spermatocytes
|
|---|---|
| Published in |
Chromosoma, March 1996
|
| DOI | 10.1007/bf00352265 |
| Pubmed ID | |
| Authors |
J. W. Allen, D. J. Dix, B. W. Collins, B. A. Merrick, C. He, J. K. Selkirk, P. Poorman-Allen, M. E. Dresser, E. M. Eddy |
| Abstract |
Mouse spermatogenic cells are known to express HSP70-2, a member of the HSP70 family of heat-shock proteins. The purpose of the present study was to characterize further the expression and localization of HSP70-2 in meiotic cells of mice and hamsters. After separating mouse spermatogenic cells into cytoplasmic and nuclear fractions, proteins were separated by two-dimensional gel electrophoresis and detected with HSP-specific antibodies. Of several HSP70 proteins identified in the cytoplasm, only HSC70 and HSP70-2 were also detected in the nucleus. Immunocytological analyses of spermatocyte prophase cells revealed that HSP70-2 was associated with the synaptonemal complex. Surface-spread synaptonemal complexes at pachytene and diplotene stages labeled distinctly with the antiserum to HSP70-2. Synaptonemal complexes from fetal mouse oocytes failed to show any evidence of HSP70-2. Reverse-transcriptase-polymerase chain reaction (RT-PCR) analyses of gene expression confirmed this sex specificity; Hsp70-2 mRNA was detected in mouse testes, but not ovaries. These findings are suggestive of a previously unsuspected sexual dimorphism in structure and/or function of the synaptonemal complex. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 6% |
| Unknown | 17 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 3 | 17% |
| Student > Master | 3 | 17% |
| Researcher | 3 | 17% |
| Student > Ph. D. Student | 3 | 17% |
| Student > Doctoral Student | 2 | 11% |
| Other | 2 | 11% |
| Unknown | 2 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 39% |
| Agricultural and Biological Sciences | 6 | 33% |
| Unspecified | 1 | 6% |
| Veterinary Science and Veterinary Medicine | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 2 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#7,454,066
of 22,788,370 outputs
Outputs from Chromosoma
#178
of 757 outputs
Outputs of similar age
#8,199
of 26,783 outputs
Outputs of similar age from Chromosoma
#1
of 2 outputs
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