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Regulated Localization of Rab18 to Lipid Droplets EFFECTS OF LIPOLYTIC STIMULATION AND INHIBITION OF LIPID DROPLET CATABOLISM *

Overview of attention for article published in Journal of Biological Chemistry, October 2005
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  • Above-average Attention Score compared to outputs of the same age and source (53rd percentile)

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1 X user
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3 Wikipedia pages

Citations

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255 Dimensions

Readers on

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136 Mendeley
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1 CiteULike
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2 Connotea
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Title
Regulated Localization of Rab18 to Lipid Droplets EFFECTS OF LIPOLYTIC STIMULATION AND INHIBITION OF LIPID DROPLET CATABOLISM *
Published in
Journal of Biological Chemistry, October 2005
DOI 10.1074/jbc.m506651200
Pubmed ID
Authors

Sally Martin, Kim Driessen, Susan J. Nixon, Marino Zerial, Robert G. Parton

Abstract

Rab GTPases are crucial regulators of membrane traffic. Here we have examined a possible association of Rab proteins with lipid droplets (LDs), neutral lipid-containing organelles surrounded by a phospholipid monolayer, also known as lipid bodies, which have been traditionally considered relatively inert storage organelles. Although we found close apposition between LDs and endosomal compartments labeled by expressed Rab5, Rab7, or Rab11 constructs, there was no detectable labeling of the LD surface itself by these Rab proteins. In contrast, GFP-Rab18 localized to LDs and immunoelectron microscopy showed direct association with the monolayer surface. Green fluorescent protein (GFP)-Rab18-labeled LDs underwent oscillatory movements in a localized area as well as sporadic, rapid, saltatory movements both in the periphery of the cell and toward the perinuclear region. In both adipocytes and non-adipocyte cell lines Rab18 localized to a subset of LDs. To gain insights into this specific localization, Rab18 was co-expressed with Cav3DGV, a truncation mutant of caveolin-3 shown to inhibit the catabolism and motility of lipid droplets. GFP-Rab18 and mRFP-Cav3DGV labeled mutually exclusive subpopulations of LDs. Moreover, in 3T3-L1 adipocytes, stimulation of lipolysis increased the localization of Rab18 to LDs, an effect reversed by beta-adrenergic antagonists. These results show that a Rab protein localizes directly to the monolayer surface of LDs. In addition, association with the LD surface was increased following stimulation of lipolysis and inhibited by a caveolin mutant suggesting that recruitment of Rab18 is regulated by the metabolic state of individual LDs.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 136 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 2%
Hungary 1 <1%
Finland 1 <1%
Germany 1 <1%
China 1 <1%
Denmark 1 <1%
Unknown 128 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 41 30%
Researcher 21 15%
Student > Master 18 13%
Student > Doctoral Student 11 8%
Student > Bachelor 7 5%
Other 20 15%
Unknown 18 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 59 43%
Biochemistry, Genetics and Molecular Biology 35 26%
Medicine and Dentistry 6 4%
Neuroscience 3 2%
Chemistry 3 2%
Other 7 5%
Unknown 23 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 October 2020.
All research outputs
#7,960,693
of 25,374,917 outputs
Outputs from Journal of Biological Chemistry
#31,416
of 85,237 outputs
Outputs of similar age
#24,224
of 71,149 outputs
Outputs of similar age from Journal of Biological Chemistry
#260
of 576 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 85,237 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 62% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 71,149 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 576 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.