| Title |
Reconstituted Micelle Formation Using Reduced, Carboxymethylated Bovine κ-Casein and Human β-Casein
|
|---|---|
| Published in |
The Protein Journal, September 2006
|
| DOI | 10.1007/s10930-006-9022-7 |
| Pubmed ID | |
| Authors |
Satish M. Sood, Tim Lekic, Harbir Jhawar, Harold M. Farrell, Charles W. Slattery |
| Abstract |
In milk, kappa-casein, a mixture of disulfide-bonded polymers, stabilizes and regulates the size of the unique colloidal complex of protein, Ca2+ and inorganic phosphate (Pi) termed the casein (CN) micelle. However, reduced, carboxymethylated bovine kappa-CN (RCM-kappa) forms fibrils at 37 degrees C and its micelle-forming ability is in question. Here, the doubly- and quadruply-phosphorylated human beta-CN forms and 1:1 (wt:wt) mixtures were combined with RCM-kappa at different beta/kappa weight ratios. Turbidity (OD(400 nm)) and a lack of precipitation up to 37 degrees C were used as an index of micelle formation. Studies were with 0, 5 and 10 mM Ca2+ and 4 and 8 mM Pi. The RCM-kappa does form concentration-dependent micelles. Also, beta-CN phosphorylation level influences micelle formation. Complexes were low-temperature reversible and RCM-kappa fibrils were seen. There appears to be equilibrium between fibrillar and soluble forms since the solution still stabilized after fibril removal. The RCM-kappa stabilized better than native bovine kappa-CN. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 29% |
| Other | 5 | 18% |
| Student > Ph. D. Student | 2 | 7% |
| Student > Doctoral Student | 2 | 7% |
| Student > Master | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 9 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 25% |
| Biochemistry, Genetics and Molecular Biology | 6 | 21% |
| Chemistry | 2 | 7% |
| Computer Science | 1 | 4% |
| Immunology and Microbiology | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 10 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#8,533,995
of 25,371,288 outputs
Outputs from The Protein Journal
#163
of 639 outputs
Outputs of similar age
#30,679
of 89,586 outputs
Outputs of similar age from The Protein Journal
#1
of 4 outputs
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