| Title |
Small Ubiquitin-Like Modifier Protein 3 Enhances the Solubilization of Human Bone Morphogenetic Protein 2 in E. coli
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, March 2018
|
| DOI | 10.1007/s12010-018-2736-0 |
| Pubmed ID | |
| Authors |
Muhammad Umair Hanif, Adnan Yaseen, Roquyya Gul, Muhammad Usman Mirza, Muhammad Hassan Nawaz, Syed Shoaib Ahmed, Salman Aziz, Saima Chaudhary, Ayyaz Ali Khan, Muhammad Shoaib |
| Abstract |
Small ubiquitin-like modifier (SUMO) fusion technology is widely used in the production of heterologous proteins from prokaryotic system to aid in protein solubilization and refolding. Due to an extensive clinical application of human bone morphogenetic protein 2 (hBMP2) in bone augmentation, total RNA was isolated from human gingival tissue and mature gene was amplified through RT-PCR, cloned (pET21a), sequence analyzed, and submitted to GenBank (Accession no. KF250425). To obtain soluble expression, SUMO3 was tagged at the N-terminus of hBMP2 gene (pET21a/SUMO3-hBMP2), transferred in BL21 codon+, and ~ 40% soluble expression was obtained on induction with IPTG. The dimerized hBMP2 was confirmed with Western blot, native PAGE analysis, and purified by fast protein liquid chromatography with 0.5 M NaCl elution. The cleavage of SUMO3 tag from hBMP2 converted it to an insoluble form. Computational 3D structural analysis of the SUMO3-hBMP2 was performed and optimized by molecular dynamic simulation. Protein-protein interaction of SUMO3-hBMP2 with BMP2 receptor was carried out using HADDOCK and inferred stable interaction. The alkaline phosphatase assay of SUMO3-hBMP2 on C2C12 cells showed maximum 200-ng/ml dose-dependent activity. We conclude that SUMO3-tagged hBMP2 is more suited for generation of soluble form of the protein and addition of SUMO3 tag does not affect the functional activity of hBMP2. |
X Demographics
The data shown below were collected from the profiles of 27 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Pakistan | 8 | 30% |
| Canada | 1 | 4% |
| United States | 1 | 4% |
| Netherlands | 1 | 4% |
| Unknown | 16 | 59% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 25 | 93% |
| Science communicators (journalists, bloggers, editors) | 1 | 4% |
| Scientists | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 16% |
| Researcher | 2 | 11% |
| Student > Bachelor | 1 | 5% |
| Student > Doctoral Student | 1 | 5% |
| Student > Postgraduate | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 11 | 58% |
| Readers by discipline | Count | As % |
|---|---|---|
| Business, Management and Accounting | 2 | 11% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Agricultural and Biological Sciences | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 12 | 63% |
Attention Score in Context
This research output has an Altmetric Attention Score of 24. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2020.
All research outputs
#1,607,178
of 25,709,917 outputs
Outputs from Applied Biochemistry and Biotechnology
#9
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Outputs of similar age
#34,524
of 347,575 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#1
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Altmetric has tracked 25,709,917 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,779 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 99% of its peers.
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We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.