| Title |
TASK-5, a novel member of the tandem pore K+ channel family
|
|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, September 2001
|
| DOI | 10.1007/s004240100620 |
| Pubmed ID | |
| Authors |
Ian Ashmole, Paul A. Goodwin, Peter R. Stanfield |
| Abstract |
We have cloned a novel member of the tandem pore K+ channel family from human brain cDNA. The novel cDNA encodes a 330-residue polypeptide of predicted molecular mass 36 kDa. We have named the channel TASK-5 owing to its sequence homology with TASK-1 and TASK-3. TASK-5 mRNA is expressed in pancreas, liver, kidney, lung, ovary, testis and heart. However, expression of TASK-5 in heterologous systems failed to elicit ionic currents. Removal of a putative endoplasmic reticulum retention sequence did not alter this finding and the distribution of channel proteins in HEK293 cells was similar for both TASK-1 and TASK-5. We tested whether TASK-5 could form heteromers with TASK-1. We show a mutant form of TASK-1 (H98N) to have a radically reduced sensitivity to acidification. Proton sensitivity could be rescued by injecting equimolar amounts of wild-type and mutant TASK-1 cRNA into Xenopus oocytes; the effect was that expected if half the channels formed are heteromers. Co-expression of TASK-5 with TASK-1 H98N does not affect the proton sensitivity of mutant TASK-1; thus TASK-5 appears not to form heteromers with TASK-1. Nonetheless, TASK-5 may require some other, unidentified partner subunit to form functional channels in the plasma membrane or it may form a channel in an intracellular organelle. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Japan | 1 | 3% |
| Mexico | 1 | 3% |
| Unknown | 29 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 25% |
| Researcher | 7 | 22% |
| Student > Doctoral Student | 3 | 9% |
| Student > Bachelor | 3 | 9% |
| Student > Master | 3 | 9% |
| Other | 5 | 16% |
| Unknown | 3 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 38% |
| Neuroscience | 5 | 16% |
| Medicine and Dentistry | 4 | 13% |
| Immunology and Microbiology | 2 | 6% |
| Biochemistry, Genetics and Molecular Biology | 1 | 3% |
| Other | 4 | 13% |
| Unknown | 4 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 September 2017.
All research outputs
#8,510,224
of 25,378,799 outputs
Outputs from Pflügers Archiv - European Journal of Physiology
#511
of 2,060 outputs
Outputs of similar age
#13,963
of 40,633 outputs
Outputs of similar age from Pflügers Archiv - European Journal of Physiology
#4
of 14 outputs
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