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Mendeley readers
Attention Score in Context
| Title |
In Vitro Modification of Human Centromere Protein CENP-C Fragments by Small Ubiquitin-like Modifier (SUMO) Protein DEFINITIVE IDENTIFICATION OF THE MODIFICATION SITES BY TANDEM MASS SPECTROMETRY ANALYSIS OF THE ISOPEPTIDES*
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|---|---|
| Published in |
Journal of Biological Chemistry, July 2004
|
| DOI | 10.1074/jbc.m405637200 |
| Pubmed ID | |
| Authors |
Tung-Liang Chung, He-Hsuan Hsiao, Yuh-Ying Yeh, Hui-Ling Shia, Yi-Ling Chen, Po-Huang Liang, Andrew H.-J. Wang, Kay-Hooi Khoo, Steven Shoei-Lung Li |
| Abstract |
Protein sumoylation by small ubiquitin-like modifier (SUMO) proteins is an important post-translational regulatory modification. A role in the control of chromosome dynamics was first suggested when SUMO was identified as high-copy suppressor of the centromere protein CENP-C mutants. CENP-C itself contains a consensus sumoylation sequence motif that partially overlaps with its DNA binding and centromere localization domain. To ascertain whether CENP-C can be sumoylated, tandem mass spectrometry (MS) based strategy was developed for high sensitivity identification and sequencing of sumoylated isopeptides present among in-gel-digested tryptic peptides of SDS-PAGE fractionated target proteins. Without a predisposition to searching for the expected isopeptides based on calculated molecular mass and relying instead on the characteristic MS/MS fragmentation pattern to identify sumolylation, we demonstrate that several other lysine residues located not within the perfect consensus sumoylation motif psiKXE/D, where psi represents a large hydrophobic amino acid, and X represents any amino acid, can be sumolylated with a reconstituted in vitro system containing only the SUMO proteins, E1-activating enzyme and E2-conjugating enzyme (Ubc9). In all cases, target sites that can be sumoylated by SUMO-2 were shown to be equally susceptible to SUMO-1 attachments which include specific sites on SUMO-2 itself, Ubc9, and the recombinant CENP-C fragments. Two non-consensus sites on one of the CENP-C fragments were found to be sumoylated in addition to the predicted site on the other fragment. The developed methodologies should facilitate future studies in delineating the dynamics and substrate specificities of SUMO-1/2/3 modifications and the respective roles of E3 ligases in the process. |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 29% |
| Student > Ph. D. Student | 7 | 20% |
| Professor > Associate Professor | 5 | 14% |
| Professor | 4 | 11% |
| Student > Doctoral Student | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 4 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 49% |
| Biochemistry, Genetics and Molecular Biology | 9 | 26% |
| Unspecified | 1 | 3% |
| Chemical Engineering | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 3 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from Journal of Biological Chemistry
#32,957
of 85,240 outputs
Outputs of similar age
#21,134
of 59,614 outputs
Outputs of similar age from Journal of Biological Chemistry
#309
of 796 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 85,240 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 59,614 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 796 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.