| Title |
Proteomic analysis of antimicrobial effects of pegylated silver coated carbon nanotubes in Salmonella enterica serovar Typhimurium
|
|---|---|
| Published in |
Journal of Nanobiotechnology, March 2018
|
| DOI | 10.1186/s12951-018-0355-0 |
| Pubmed ID | |
| Authors |
Seong B. Park, Christy S. Steadman, Atul A. Chaudhari, Shreekumar R. Pillai, Shree R. Singh, Peter L. Ryan, Scott T. Willard, Jean M. Feugang |
| Abstract |
Synthesis of silver nano-compounds with enhanced antimicrobial effects is of great interest for the development of new antibacterial agents. Previous studies have reported the antibacterial properties of pegylated silver-coated carbon nanotubes (pSWCNT-Ag) showing less toxicity in human cell lines. However, the mechanism underlining the pSWCNT-Ag as a bactericidal agent remained unfolded. Here we assessed the pSWCNT-Ag effects against foodborne pathogenic bacteria growth and proteome profile changes. Measurements of bioluminescent imaging, optical density, and bacteria colony forming units revealed dose-dependent and stronger bactericidal activity of pSWCNT-Ag than their non-pegylated counterparts (SWCNT-Ag). In ovo administration of pSWCNT-Ag or phosphate-buffered saline resulted in comparable chicken embryo development and growth. The proteomic analysis, using two-dimensional electrophoresis combined with matrix assisted laser desorption/ionization time of flight/time of flight mass spectrometry, was performed on control and surviving Salmonella enterica serovar Typhimurium to pSWCNT-Ag. A total of 15 proteins (ten up-regulated and five down-regulated) differentially expressed proteins were identified. Functional analyses showed significant reduction of proteins associated with biofilm formation, nutrient and energy metabolism, quorum sensing and maintenance of cell structure and cell motility in surviving S. Typhimurium. In contrast, proteins associated with oxygen stress, DNA protection, starvation, membrane rebuilding, and alternative nutrient formation were induced as the compensatory reaction. This study provides further evidence of the antibacterial effects of pSWCNT-Ag nanocomposites and knowledge of their mechanism of action through various protein changes. The findings may lead to the development of more effective and safe antimicrobial agents. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 67 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 15% |
| Researcher | 8 | 12% |
| Student > Master | 6 | 9% |
| Student > Bachelor | 5 | 7% |
| Student > Doctoral Student | 2 | 3% |
| Other | 10 | 15% |
| Unknown | 26 | 39% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 12% |
| Chemistry | 7 | 10% |
| Biochemistry, Genetics and Molecular Biology | 6 | 9% |
| Immunology and Microbiology | 4 | 6% |
| Social Sciences | 3 | 4% |
| Other | 12 | 18% |
| Unknown | 27 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2018.
All research outputs
#17,937,475
of 23,031,582 outputs
Outputs from Journal of Nanobiotechnology
#795
of 1,440 outputs
Outputs of similar age
#239,780
of 330,033 outputs
Outputs of similar age from Journal of Nanobiotechnology
#15
of 24 outputs
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So far Altmetric has tracked 1,440 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
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