Title |
Cryopreserved clumps of mesenchymal stem cell/extracellular matrix complexes retain osteogenic capacity and induce bone regeneration
|
---|---|
Published in |
Stem Cell Research & Therapy, March 2018
|
DOI | 10.1186/s13287-018-0826-0 |
Pubmed ID | |
Authors |
Souta Motoike, Mikihito Kajiya, Nao Komatsu, Manabu Takewaki, Susumu Horikoshi, Shinji Matsuda, Kazuhisa Ouhara, Tomoyuki Iwata, Katsuhiro Takeda, Tsuyoshi Fujita, Hidemi Kurihara |
Abstract |
Three-dimensional (3D) cultured clumps of mesenchymal stem cell (MSC)/extracellular matrix (ECM) complexes (C-MSCs) consist of cells and self-produced ECM. C-MSCs can regulate cellular functions in vitro and can be grafted into a defect site without an artificial scaffold to induce bone regeneration. Long-term cryopreservation of C-MSCs, which can enable them to serve as a ready-to-use cell preparation, may be helpful in developing beneficial cell therapy for bone regeneration. Therefore, the aim of this study was to investigate the effect of cryopreservation on C-MSCs. MSCs isolated from rat femurs were cultured in growth medium supplemented with ascorbic acid. To obtain C-MSCs, confluent cells that had formed on the cellular sheet were scratched using a micropipette tip and were then torn off. The sheet was rolled to make a round clumps of cells. The C-MSCs were cryopreserved in cryomedium including 10% dimethyl sulfoxide. Cryopreserved C-MSCs retained their 3D structure and did not exhibit a decrease in cell viability. In addition, stem cell marker expression levels and the osteogenic differentiation properties of C-MSCs were not reduced by cryopreservation. However, C-MSCs pretreated with collagenase before cryopreservation showed a lower level of type I collagen and could not retain their 3D structure, and their rates of cell death increased during cryopreservation. Both C-MSC and cryopreserved C-MSC transplantation into rat calvarial defects induced successful bone regeneration. These data indicate that cryopreservation does not reduce the biological properties of C-MSCs because of its abundant type I collagen. More specifically, cryopreserved C-MSCs could be applicable for novel bone regenerative therapies. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
South Africa | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 33% |
Scientists | 1 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 50 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 14% |
Researcher | 5 | 10% |
Student > Master | 5 | 10% |
Student > Postgraduate | 4 | 8% |
Student > Bachelor | 3 | 6% |
Other | 7 | 14% |
Unknown | 19 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 15 | 30% |
Engineering | 3 | 6% |
Agricultural and Biological Sciences | 3 | 6% |
Biochemistry, Genetics and Molecular Biology | 2 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 3 | 6% |
Unknown | 23 | 46% |