| Title |
A novel agent, methylophiopogonanone B, promotes Rho activation and tubulin depolymerization
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, October 2006
|
| DOI | 10.1007/s11010-006-9336-y |
| Pubmed ID | |
| Authors |
Yuko Ito, Akiko Kanamaru, Akihiro Tada |
| Abstract |
Cytoskeletal reorganization, including reconstruction of actin fibers and microtubules, is essential for various biological processes, such as cell migration, proliferation and dendrite formation. We show here that methylophiopogonanone B (MOPB) induces cell morphological change via melanocyte dendrite retraction and stress fiber formation. Since members of the Rho family of small GTP-binding proteins act as master regulators of dendrite formation and actin cytoskeletal reorganization, and activated Rho promotes dendrite retraction and stress fiber formation, we studied the effects of MOPB on the small GTPases using normal human epidermal melanocytes and HeLa cells. In in vitro binding assay, MOPB significantly increased GTP-Rho, but not GTP-Rac or GTP-CDC42. Furthermore, a Rho inhibitor, a Rho kinase inhibitor and a small GTPase inhibitor each blocked MOPB-induced stress fiber formation. The effect of MOPB on actin reorganization was blocked in a Rho dominant negative mutant. These results suggest MOPB acts via the Rho signaling pathway, and it may directly or indirectly activate Rho. Quantitative Western blot analysis indicated that MOPB also induced microtubule destabilization and tubulin depolymerization. Thus, MOPB appears to induce Rho activation, resulting in actin cytoskeletal reorganization, including dendrite retraction and stress fiber formation. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 8% |
| Poland | 1 | 8% |
| Unknown | 10 | 83% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 42% |
| Student > Bachelor | 2 | 17% |
| Other | 2 | 17% |
| Student > Ph. D. Student | 1 | 8% |
| Professor | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Agricultural and Biological Sciences | 3 | 25% |
| Medicine and Dentistry | 2 | 17% |
| Sports and Recreations | 1 | 8% |
| Chemistry | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 December 2007.
All research outputs
#7,454,066
of 22,788,370 outputs
Outputs from Molecular and Cellular Biochemistry
#417
of 2,301 outputs
Outputs of similar age
#23,234
of 66,535 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#2
of 15 outputs
Altmetric has tracked 22,788,370 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,301 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 50% of its peers.
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