| Title |
Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay
|
|---|---|
| Published in |
Microchimica Acta, December 2017
|
| DOI | 10.1007/s00604-017-2550-3 |
| Pubmed ID | |
| Authors |
Najmeh Ansari, Kiarash Ghazvini, Mohammad Ramezani, Mahin Shahdordizadeh, Rezvan Yazdian-Robati, Khalil Abnous, Seyed Mohammad Taghdisi |
| Abstract |
The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. It is a potential marker for early diagnosis of tuberculosis (TB). The authors have identified specific aptamers for Ag85A (FbpA) via protein SELEX using magnetic beads. After twelve rounds of selection, two aptamers (Apt8 and Apt22) were chosen from different groups, and their binding constants were determined by flow cytometry. Apt22 (labeled with Atto 647N) binds to FbpA with high affinity (Kd = 63 nM) and specificity. A rapid, sensitive, and low-cost fluorescent assay was designed based on the use of Apt22 and graphene oxide, with a limit of detection of 1.5 nM and an analytical range from 5 to 200 nM of FbpA. Graphical abstract Schematic illustration of graphene oxide-based aptasensor for fluorometric determination of FbpA. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 45 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 18% |
| Unspecified | 7 | 16% |
| Student > Ph. D. Student | 5 | 11% |
| Student > Doctoral Student | 3 | 7% |
| Professor | 3 | 7% |
| Other | 7 | 16% |
| Unknown | 12 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 7 | 16% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Medicine and Dentistry | 3 | 7% |
| Chemistry | 3 | 7% |
| Agricultural and Biological Sciences | 2 | 4% |
| Other | 9 | 20% |
| Unknown | 16 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2018.
All research outputs
#13,509,734
of 23,031,582 outputs
Outputs from Microchimica Acta
#572
of 1,402 outputs
Outputs of similar age
#214,875
of 439,675 outputs
Outputs of similar age from Microchimica Acta
#14
of 29 outputs
Altmetric has tracked 23,031,582 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,402 research outputs from this source. They receive a mean Attention Score of 2.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 439,675 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.