| Title |
A sample-to-answer droplet magnetofluidic assay platform for quantitative methylation-specific PCR
|
|---|---|
| Published in |
Biomedical Microdevices, March 2018
|
| DOI | 10.1007/s10544-018-0276-6 |
| Pubmed ID | |
| Authors |
Alejandro Stark, Dong Jin Shin, Tza-Huei Wang |
| Abstract |
Dysregulation of DNA methylation has been identified as an epigenetic biomarker for numerous cancer types. Gene-specific identification techniques relying on methylation-specific PCR (MSP) require a lengthy manual benchtop process that is susceptible to human-error and contamination. This MSP assay requires a series of discrete sample processing steps including genomic DNA extraction, bisulfite conversion and readout via PCR. In this work, we present a streamlined assay platform utilizing droplet magnetofluidic principles for integration of all sample processing steps required to obtain quantitative MSP signal from raw biological samples. We present a streamlined protocol for solid-phase extraction and bisulfite conversion of genomic DNA, which minimizes reagent use and simplifies the sample preparation protocol for implementation on a compact assay platform. Furthermore, we present a thermally robust assay chip that enables DNA extraction, bisulfite conversion and quantitative PCR from biological samples on a single device. Technical improvements to facilitate DNA extraction and PCR on a single chip in addition to chip performance characterization data are presented. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 35% |
| Student > Bachelor | 3 | 15% |
| Researcher | 2 | 10% |
| Student > Master | 1 | 5% |
| Unknown | 7 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 7 | 35% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Nursing and Health Professions | 1 | 5% |
| Chemical Engineering | 1 | 5% |
| Materials Science | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 7 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2019.
All research outputs
#6,220,174
of 23,031,582 outputs
Outputs from Biomedical Microdevices
#200
of 755 outputs
Outputs of similar age
#109,589
of 329,889 outputs
Outputs of similar age from Biomedical Microdevices
#4
of 7 outputs
Altmetric has tracked 23,031,582 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 755 research outputs from this source. They receive a mean Attention Score of 4.1. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,889 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.