| Title |
Comparing human peritoneal fluid and phosphate-buffered saline for drug delivery: do we need bio-relevant media?
|
|---|---|
| Published in |
Drug Delivery and Translational Research, March 2018
|
| DOI | 10.1007/s13346-018-0513-9 |
| Pubmed ID | |
| Authors |
Prabhat Bhusal, Jamie Lee Rahiri, Bruce Sua, Jessica E. McDonald, Mahima Bansal, Sara Hanning, Manisha Sharma, Kaushik Chandramouli, Jeff Harrison, Georgina Procter, Gavin Andrews, David S. Jones, Andrew G. Hill, Darren Svirskis |
| Abstract |
An understanding of biological fluids at the site of administration is important to predict the fate of drug delivery systems in vivo. Little is known about peritoneal fluid; therefore, we have investigated this biological fluid and compared it to phosphate-buffered saline, a synthetic media commonly used for in vitro evaluation of intraperitoneal drug delivery systems. Human peritoneal fluid samples were analysed for electrolyte, protein and lipid levels. In addition, physicochemical properties were measured alongside rheological parameters. Significant inter-patient variations were observed with regard to pH (p < 0.001), buffer capacity (p < 0.05), osmolality (p < 0.001) and surface tension (p < 0.05). All the investigated physicochemical properties of peritoneal fluid differed from phosphate-buffered saline (p < 0.001). Rheological examination of peritoneal fluid demonstrated non-Newtonian shear thinning behaviour and predominantly exhibited the characteristics of an entangled network. Inter-patient and inter-day variability in the viscosity of peritoneal fluid was observed. The solubility of the local anaesthetic lidocaine in peritoneal fluid was significantly higher (p < 0.05) when compared to phosphate-buffered saline. Interestingly, the dissolution rate of lidocaine was not significantly different between the synthetic and biological media. Importantly, and with relevance to intraperitoneal drug delivery systems, the sustained release of lidocaine from a thermosensitive gel formulation occurred at a significantly faster rate into peritoneal fluid. Collectively, these data demonstrate the variation between commonly used synthetic media and human peritoneal fluid. The differences in drug release rates observed illustrate the need for bio-relevant media, which ultimately would improve in vitro-in vivo correlation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 19% |
| Other | 5 | 14% |
| Student > Bachelor | 5 | 14% |
| Researcher | 4 | 11% |
| Student > Master | 3 | 8% |
| Other | 7 | 19% |
| Unknown | 6 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 11 | 30% |
| Chemistry | 5 | 14% |
| Engineering | 3 | 8% |
| Medicine and Dentistry | 3 | 8% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 8 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2018.
All research outputs
#15,498,204
of 23,031,582 outputs
Outputs from Drug Delivery and Translational Research
#326
of 516 outputs
Outputs of similar age
#210,834
of 330,380 outputs
Outputs of similar age from Drug Delivery and Translational Research
#8
of 12 outputs
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