| Title |
Serum leucine-rich alpha-2-glycoprotein-1 binds cytochrome c and inhibits antibody detection of this apoptotic marker in enzyme-linked immunosorbent assay
|
|---|---|
| Published in |
Apoptosis, May 2006
|
| DOI | 10.1007/s10495-006-8159-3 |
| Pubmed ID | |
| Authors |
Chad Cummings, Jennifer Walder, Amy Treeful, Ronald Jemmerson |
| Abstract |
Cytochrome c (Cyt c) has been implicated as a serum marker for aberrant apoptosis and, thus, has considerable clinical potential. Using a sandwich enzyme-linked immunosorbent assay (ELISA) we found that the sensitivity of Cyt c detection is reduced in the presence of serum. The inhibitory factor responsible was purified from both fetal bovine serum and human serum employing standard chromatography procedures followed by affinity chromatography on Affi-Gel 10-bound Cyt c. In SDS-PAGE, bands at 44 kD and 50 kD were observed for the bovine and human proteins, respectively. Mass spectrometry analysis identified the serum inhibitory factor as leucine-rich alpha-2-glycoprotein-1 (LRalpha2GP1). This identification may lead to a modified ELISA to quantify total Cyt c in patients' sera. LRalpha2GP1 is the first extracellular ligand for Cyt c that has been identified. A physiological function associated with binding is suggested. |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 2 | 7% |
| Unknown | 27 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 24% |
| Student > Ph. D. Student | 6 | 21% |
| Student > Bachelor | 5 | 17% |
| Student > Master | 3 | 10% |
| Student > Doctoral Student | 1 | 3% |
| Other | 4 | 14% |
| Unknown | 3 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 38% |
| Biochemistry, Genetics and Molecular Biology | 5 | 17% |
| Medicine and Dentistry | 5 | 17% |
| Immunology and Microbiology | 1 | 3% |
| Unspecified | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 4 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 June 2023.
All research outputs
#4,696,560
of 22,788,370 outputs
Outputs from Apoptosis
#70
of 804 outputs
Outputs of similar age
#11,944
of 66,093 outputs
Outputs of similar age from Apoptosis
#1
of 8 outputs
Altmetric has tracked 22,788,370 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 804 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 66,093 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them