| Title |
Identification and rapid mapping of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA capture technologies
|
|---|---|
| Published in |
Theoretical and Applied Genetics, March 2018
|
| DOI | 10.1007/s00122-018-3078-6 |
| Pubmed ID | |
| Authors |
Xinwei Chen, Dominika Lewandowska, Miles R. Armstrong, Katie Baker, Tze-Yin Lim, Micha Bayer, Brian Harrower, Karen McLean, Florian Jupe, Kamil Witek, Alison K. Lees, Jonathan D. Jones, Glenn J. Bryan, Ingo Hein |
| Abstract |
A broad-spectrum late blight disease-resistance gene from Solanum verrucosum has been mapped to potato chromosome 9. The gene is distinct from previously identified-resistance genes. We have identified and characterised a broad-spectrum resistance to Phytophthora infestans from the wild Mexican species Solanum verrucosum. Diagnostic resistance gene enrichment (dRenSeq) revealed that the resistance is not conferred by previously identified nucleotide-binding, leucine-rich repeat genes. Utilising the sequenced potato genome as a reference, two complementary enrichment strategies that target resistance genes (RenSeq) and single/low-copy number genes (Generic-mapping enrichment Sequencing; GenSeq), respectively, were deployed for the rapid, SNP-based mapping of the resistance through bulked-segregant analysis. Both approaches independently positioned the resistance, referred to as Rpi-ver1, to the distal end of potato chromosome 9. Stringent post-enrichment read filtering identified a total of 64 informative SNPs that corresponded to the expected ratio for significant polymorphisms in the parents as well as the bulks. Of these, 61 SNPs are located on potato chromosome 9 and reside within 27 individual genes, which in the sequenced potato clone DM locate to positions 45.9 to 60.9 Mb. RenSeq- and GenSeq-derived SNPs within the target region were converted into allele-specific PCR-based KASP markers and further defined the position of the resistance to a 4.3 Mb interval at the bottom end of chromosome 9 between positions 52.62-56.98 Mb. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 6 | 17% |
| Germany | 3 | 8% |
| Netherlands | 3 | 8% |
| United States | 3 | 8% |
| Australia | 2 | 6% |
| Thailand | 1 | 3% |
| Canada | 1 | 3% |
| Italy | 1 | 3% |
| France | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 14 | 39% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 24 | 67% |
| Scientists | 8 | 22% |
| Science communicators (journalists, bloggers, editors) | 4 | 11% |
Mendeley readers
The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 97 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 29 | 30% |
| Student > Ph. D. Student | 18 | 19% |
| Student > Master | 9 | 9% |
| Student > Bachelor | 7 | 7% |
| Student > Doctoral Student | 4 | 4% |
| Other | 8 | 8% |
| Unknown | 22 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 57 | 59% |
| Biochemistry, Genetics and Molecular Biology | 16 | 16% |
| Medicine and Dentistry | 1 | 1% |
| Engineering | 1 | 1% |
| Unknown | 22 | 23% |
Attention Score in Context
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Outputs from Theoretical and Applied Genetics
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Outputs of similar age from Theoretical and Applied Genetics
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