| Title |
Gene Expression of runx2, Osterix, c-fos, DLX-3, DLX-5, and MSX-2 in Dental Follicle Cells during Osteogenic Differentiation In Vitro
|
|---|---|
| Published in |
Calcified Tissue International, February 2006
|
| DOI | 10.1007/s00223-005-0146-0 |
| Pubmed ID | |
| Authors |
C. Morsczeck |
| Abstract |
Recently, osteogenic precursor cells were isolated from human dental follicles, which differentiate into cementoblast- or osteoblast- like cells under in vitro conditions. However, mechanisms for osteogenic differentiation are not known in detail. Dental follicle cell long-term cultures supplemented with dexamethasone or with insulin resulted in mineralized nodules, whereas no mineralization or alkaline phosphatase activity was detected in the control culture without an osteogenic stimulus. A real-time reverse-transcriptase polymerase chain reaction (PCR) analysis was developed to investigate gene expression during osteogenic differentiation in vitro. Expression of the alkaline phosphatase (ALP) gene was detected during differentiation in the control culture and was similar to that in cultures with dexamethasone and insulin. DLX-3, DLX-5, runx2, and MSX-2 are differentially expressed during osteogenic differentiation in bone marrow mesenchymal stem cells. In dental follicle cells, gene expression of runx2, DLX-5, and MSX-2 was unaffected during osteogenic differentiation in vitro. Osteogenic differentiation appeared to be independent of MSX-2 expression; the same was true of runx2 and DLX-5, which were protagonists of osteogenic differentiation and osteocalcin promoter activity in bone marrow mesenchymal stem cells. Like in bone marrow-derived stem cells, DLX-3 gene expression was increased in dental follicle cells during osteogenic differentiation but similar to control cultures. However, gene expression of osterix was not detected in dental follicle cells during osteogenic differentiation; this gene is expressed during osteogenic differentiation in bone marrow stem cells. These real-time PCR results display molecular mechanisms in dental follicle precursor cells during osteogenic differentiation that are different from those in bone marrow-derived mesenchymal stem cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| United States | 1 | 2% |
| Germany | 1 | 2% |
| France | 1 | 2% |
| Unknown | 46 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 22% |
| Researcher | 11 | 22% |
| Student > Postgraduate | 4 | 8% |
| Student > Master | 4 | 8% |
| Student > Bachelor | 3 | 6% |
| Other | 12 | 24% |
| Unknown | 5 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 34% |
| Medicine and Dentistry | 8 | 16% |
| Biochemistry, Genetics and Molecular Biology | 6 | 12% |
| Engineering | 3 | 6% |
| Unspecified | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 10 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#7,454,066
of 22,788,370 outputs
Outputs from Calcified Tissue International
#542
of 1,756 outputs
Outputs of similar age
#40,652
of 154,733 outputs
Outputs of similar age from Calcified Tissue International
#2
of 2 outputs
Altmetric has tracked 22,788,370 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,756 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.9. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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