Title |
Molecular Cloning and Expression of a cDNA Encoding a Human Thioredoxin-like Protein
|
---|---|
Published in |
Biochemical & Biophysical Research Communications, February 1998
|
DOI | 10.1006/bbrc.1997.8003 |
Pubmed ID | |
Authors |
Antonio Miranda-Vizuete, Jan-Åke Gustafsson, Giannis Spyrou |
Abstract |
This report describes the cloning of a human cDNA that encodes a new protein (Txl, Thioredoxin-like) that belongs to the expanding family of thioredoxins based on sequence comparison of the deduced amino acid sequence. This cDNA, with a total length of 1,278 bp, consists of 205 bp of 5'-untranslated sequence (including an in frame stop codon), an open reading frame of 870 bp and a 203 bp fragment of 3'-untranslated sequence. The coding sequence predicts a protein of 289 amino acids with two distinct domains: an N-terminal domain of 105 residues homologous to the rest of mammalian thioredoxins containing the conserved active site (CGPC) and a C-terminal domain of 184 residues with no homology with any other protein in the database. Northern blot analysis indicates that the txl probe hybridizes to a 1.3 Kb mRNA and is ubiquitously expressed in human tissues with the highest expression in stomach, testis and bone marrow. |
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