| Title |
Intracellular distribution of fatty alcohol oxidase activity in Mucor circinelloides YR-1 isolated from petroleum contaminated soils
|
|---|---|
| Published in |
Antonie van Leeuwenhoek, July 2009
|
| DOI | 10.1007/s10482-009-9368-x |
| Pubmed ID | |
| Authors |
Hortencia Silva-Jiménez, Vanesa Zazueta-Novoa, Arelí Durón-Castellanos, Carmen Rodríguez-Robelo, Carlos A. Leal-Morales, Roberto Zazueta-Sandoval |
| Abstract |
In previous studies, Mucor circinelloides YR-1 isolated from petroleum-contaminated soils grown in decane as sole carbon source, showed fatty alcohol oxidase (FAO) activities in either particulate or soluble fractions from a cell-free extract. One is associated to internal membranes (mFAO) and the other one is soluble (sFAO). Both activities appear to be located in the cells in specific compartments other than peroxisomes. Results suggested that mFAO could be located on the inner face of the membrane of these compartments, and sFAO could be in the lumen of the specific compartments. This study reports on the intracellular distribution of FAO activity and the purification of sFAOs and mFAO after several different procedures for release from the membranous fraction using the mixed membrane fraction (MMF) after cellular homogenization as enzymatic source. Results with the purified mFAO show, by molecular weight criteria, that the enzyme has only one type of subunit with molecular mass of 46 kDa, with two isoelectric point components: 6.0 and 6.3. We found that mFAO is strongly associated to the MMF, possibly in a transitory fashion. Using non-denaturating gels, we suggest that sFAO and mFAO have the same subunits in their native structures, and, due to their native molecular weight of approximately 350 kDa, each could be natively structured as an octameric complex. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 63% |
| Other | 1 | 13% |
| Student > Doctoral Student | 1 | 13% |
| Student > Master | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Immunology and Microbiology | 1 | 13% |
| Engineering | 1 | 13% |
| Unknown | 1 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 March 2012.
All research outputs
#7,454,066
of 22,788,370 outputs
Outputs from Antonie van Leeuwenhoek
#534
of 2,023 outputs
Outputs of similar age
#37,109
of 110,351 outputs
Outputs of similar age from Antonie van Leeuwenhoek
#5
of 7 outputs
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So far Altmetric has tracked 2,023 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
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