| Title |
Genomic changes in an attenuated genotype I Japanese encephalitis virus and comparison with virulent parental strain
|
|---|---|
| Published in |
Virus Genes, March 2018
|
| DOI | 10.1007/s11262-018-1559-y |
| Pubmed ID | |
| Authors |
Yuyong Zhou, Rui Wu, Yao Feng, Qin Zhao, Xintian Wen, Xiaobo Huang, Yiping Wen, Qigui Yan, Yong Huang, Xiaoping Ma, Xinfeng Han, Sanjie Cao |
| Abstract |
Genotype I Japanese encephalitis virus (JEV) strain SCYA201201 was previously isolated from brain tissues of aborted piglets. In this study, we obtained an attenuated SCYA201201-0901 strain by serial passage of strain SCYA201201-1 in Syrian baby hamster kidney cells, combined with multiple plaque purifications and selection for virulence in mice. We investigated the genetic changes associated with attenuation by comparing the entire genomes of SCYA201201-0901 and SCYA201201-1. Sequence comparisons identified 14 common amino acid substitutions in the coding region, with two nucleotide point mutations in the 5'-untranslated region (UTR) and another three in the 3'-UTR, which differed between the attenuated and virulent strains. In addition, a total of 13 silent nucleotide mutations were found after attenuation. These substitutions, alone or in combination, may be responsible for the attenuated phenotype of the SCYA201201-0901 strain in mice. This information will contribute to our understanding of attenuation and of the molecular basis of virulence in genotype I strains such as SCYA201201-0901, as well as aiding the development of safer JEV vaccines. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 25% |
| Student > Ph. D. Student | 2 | 25% |
| Student > Bachelor | 1 | 13% |
| Student > Doctoral Student | 1 | 13% |
| Professor | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 25% |
| Arts and Humanities | 1 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
| Nursing and Health Professions | 1 | 13% |
| Other | 1 | 13% |
| Unknown | 1 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2018.
All research outputs
#17,937,475
of 23,031,582 outputs
Outputs from Virus Genes
#632
of 966 outputs
Outputs of similar age
#239,812
of 329,958 outputs
Outputs of similar age from Virus Genes
#8
of 16 outputs
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