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Expression of multilectin receptors and comparative FITC–dextran uptake by human dendritic cells

Overview of attention for article published in International Immunology, November 2000
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Title
Expression of multilectin receptors and comparative FITC–dextran uptake by human dendritic cells
Published in
International Immunology, November 2000
DOI 10.1093/intimm/12.11.1511
Pubmed ID
Authors

M Kato, T K Neil, D B Fearnley, A D McLellan, S Vuckovic, D N Hart

Abstract

Dendritic cells (DC) are potent antigen-presenting cells and understanding their mechanisms of antigen uptake is important for loading DC with antigen for immunotherapy. The multilectin receptors, DEC-205 and macrophage mannose receptor (MMR), are potential antigen-uptake receptors; therefore, we examined their expression and FITC-dextran uptake by various human DC preparations. The RT-PCR analysis detected low levels of DEC-205 mRNA in immature blood DC, Langerhans cells (LC) and immature monocyte-derived DC (Mo-DC). Its mRNA expression increased markedly upon activation, indicating that DEC-205 is an activation-associated molecule. In Mo-DC, the expression of cell-surface DEC-205 increased markedly during maturation. In blood DC, however, the cell-surface expression of DEC-205 did not change during activation, suggesting the presence of a large intracellular pool of DEC-205 or post-transcriptional regulation. Immature Mo-DC expressed abundant MMR, but its expression diminished upon maturation. Blood DC and LC did not express detectable levels of the MMR. FITC-dextran uptake by both immature and activated blood DC was 30- to 70-fold less than that of LC, immature Mo-DC and macrophages. In contrast to immature Mo-DC, the FITC-dextran uptake by LC was not inhibited effectively by mannose, an inhibitor for MMR-mediated FITC-dextran uptake. Thus, unlike Mo-DC, blood DC and LC do not use the MMR for carbohydrate-conjugated antigen uptake and alternative receptors may yet be defined on these DC. Therefore, DEC-205 may have a different specificity as an antigen uptake receptor or contribute to an alternative DC function.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 2%
Germany 1 1%
France 1 1%
Netherlands 1 1%
United Kingdom 1 1%
Australia 1 1%
Unknown 82 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 26%
Researcher 20 22%
Student > Bachelor 10 11%
Student > Master 9 10%
Student > Doctoral Student 6 7%
Other 9 10%
Unknown 12 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 37 42%
Immunology and Microbiology 13 15%
Biochemistry, Genetics and Molecular Biology 9 10%
Medicine and Dentistry 9 10%
Veterinary Science and Veterinary Medicine 3 3%
Other 7 8%
Unknown 11 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2022.
All research outputs
#8,534,976
of 25,373,627 outputs
Outputs from International Immunology
#683
of 1,944 outputs
Outputs of similar age
#13,812
of 41,051 outputs
Outputs of similar age from International Immunology
#7
of 16 outputs
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We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.