| Title |
Immunogenicity of a Fap2 peptide mimotope of Fusobacterium nucleatum and its potential use in the diagnosis of colorectal cancer
|
|---|---|
| Published in |
Infectious Agents and Cancer, April 2018
|
| DOI | 10.1186/s13027-018-0184-7 |
| Pubmed ID | |
| Authors |
Leonardo A. Guevarra, Andrea Claudine F. Afable, Patricia Joyce O. Belza, Karen Joy S. Dy, Scott Justin Q. Lee, Teresa T. Sy-Ortin, Pia Marie S. P. Albano |
| Abstract |
The role ofFusobacterium nucleatumFap2 protein in the development of colorectal cancer has recently been explained. Fap2, when bound to the human inhibitory receptor, TIGIT, inhibits the cytotoxic activity of natural killer (NK) cells against cancer cells, thus, allowing proliferation of the latter eventually leading to tumor growth. The aim of the study was to identify the immunogenicity of a peptide mimotope of the Fap2 protein and to determine the reactivity of colorectal cancer patients' sera against the mimotope. Immunogenic epitope of the Fap2 protein ofF. nucleatumwas selected using the B-cell epitope prediction of the Immune Epitope Database and Analysis Resource (IEDB). The immunogenicity of the synthetic peptide mimotope of the Fap2 protein was determined in animal models and reactivity of colorectal cancer patients' sera against the mimotope was done by indirect ELISA. Results show that the selected peptide mimotope, with sequence TELAYKHYFGT, of the outer membrane protein Fap2 ofF. nucleatumis immunogenic. Increase in the absorbance readings of peptide-immunized rabbit sera was observed starting Week 1 which was sustained up to Week 10 in the indirect ELISA performed. Colorectal cancer cases (n = 37) were all reactive in an ELISA-based analysis using the mimotope as the capture antigen. In this study, we identified an immunogenic epitope of the Fap2 protein of theFusobacterium nucleatum. We demonstrated the reactivity of serum of histopathologically confirmed CRC patients in a peptide-capture indirect ELISA which may serve as proof of concept for the development of CRC diagnostics. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 55 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 14 | 25% |
| Student > Ph. D. Student | 6 | 11% |
| Student > Doctoral Student | 5 | 9% |
| Researcher | 5 | 9% |
| Student > Master | 4 | 7% |
| Other | 6 | 11% |
| Unknown | 15 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 31% |
| Medicine and Dentistry | 9 | 16% |
| Immunology and Microbiology | 7 | 13% |
| Agricultural and Biological Sciences | 4 | 7% |
| Unspecified | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 16 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 April 2018.
All research outputs
#18,594,219
of 23,031,582 outputs
Outputs from Infectious Agents and Cancer
#349
of 522 outputs
Outputs of similar age
#255,544
of 328,940 outputs
Outputs of similar age from Infectious Agents and Cancer
#6
of 6 outputs
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