| Title |
Juxtamembrane localization of the protein phosphatase-1 inhibitor protein PHI-1 in smooth muscle cells
|
|---|---|
| Published in |
Histochemistry and Cell Biology, April 2004
|
| DOI | 10.1007/s00418-004-0642-8 |
| Pubmed ID | |
| Authors |
Nikolaos A. Tountas, James W. Mandell, Allen D. Everett, David L. Brautigan |
| Abstract |
Protein phosphorylation regulates many fundamental processes and protein phosphatase-1 (PP1) is a major phosphatase that determines the levels of Ser/Thr phosphorylation. Regulatory subunits and inhibitor phosphoproteins control PP1 activity. PHI-1 is a member of a family of PP1 inhibitor phosphoproteins that was discovered based on sequence similarity to the known inhibitor CPI-17. To learn more about PHI-1 we determined the tissue distribution of PHI-1 in embryonic and adult tissues, and examined its cellular localization by immunohistochemistry. In the embryo PHI-1 appeared first in the heart at E10, and by E15 it was detected in multiple tissues. Expression in adult tissues was strikingly different, with PHI-1 detected primarily in smooth muscles in the intestine, blood vessels, and male and female genitourinary tracts. PHI-1 also was highly expressed in the endothelial layer of blood vessels. Both PHI-1 and CPI-17 are expressed predominantly in adult smooth muscles. Whereas CPI-17 staining was diffuse PHI-1 was concentrated along the cell membrane in distinct foci, detected by confocal and electron microscopy. The common tissue distribution but different cellular localization of PHI-1 and CPI-17 suggest distinctive physiological roles for these two PP1 inhibitors. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 8% |
| Unknown | 11 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 42% |
| Student > Ph. D. Student | 2 | 17% |
| Professor > Associate Professor | 2 | 17% |
| Student > Master | 1 | 8% |
| Student > Postgraduate | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 33% |
| Medicine and Dentistry | 2 | 17% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Computer Science | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 January 2008.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from Histochemistry and Cell Biology
#306
of 1,236 outputs
Outputs of similar age
#21,122
of 62,540 outputs
Outputs of similar age from Histochemistry and Cell Biology
#3
of 8 outputs
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So far Altmetric has tracked 1,236 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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