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The Nucleic Acid Protocols Handbook

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Cover of 'The Nucleic Acid Protocols Handbook'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter -1592590378 Isolation of High-Molecular-Weight DNA from Animal Cells
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    Chapter -1592590372 Isolation of mRNA by Affinity Chromatography
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    Chapter -1592590368 Isolation and Purification of DNA from Plants
  5. Altmetric Badge
    Chapter -1592590364 Purification of Uncontaminated, Intact Plant RNA
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    Chapter -1592590358 An Improved Method to Isolate Mitochondrial RNA from Green Plant Tissue
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    Chapter -1592590352 Isolating Chromosomal DNA from Bacteria
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    Chapter -1592590348 Bacterial DNA Extraction for Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis
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    Chapter -1592590344 Isolation of Fungal Nucleic Acids
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    Chapter -1592590334 Total RNA Isolation from Bacteria
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    Chapter -1592590328 Simultaneous RNA and DNA Extraction from Biopsy Material, Culture Cells, Plants, and Bacteria
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    Chapter -1592590324 Spectrophotometric Analysis of Nucleic Acids
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    Chapter -1592590318 Restriction Endonuclease Digestion of DNA
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    Chapter -1592590314 Agarose Gel Electrophoresis of Nucleic Acids
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    Chapter -1592590310 Preparation of RNA Dot Blots
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    Chapter -1592590308 Native Polyacrylamide Gel Electrophoresis
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    Chapter -1592590304 Southern Blotting of Agarose Gels by Capillary Transfer
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    Chapter -1592590300 Pulsed-Field Gel Electrophoresis
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    Chapter -1592590276 HPLC of DNA and PCR Products
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    Chapter -1592590264 End-Labeling of DNA Fragments
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    Chapter -1592590258 Nick Translation and Random Hexamer Labeling of DNA
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    Chapter -1592590254 Generation of Labeled Probes by Polymerase Chain Reaction
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    Chapter -1592590246 Nonradioactive Oligonucleotide Probe Labeling
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    Chapter -1592590236 Preparation of Direct, Enzyme-Labeled DNA Probes
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    Chapter -1592590232 Random Prime Labeling of DNA Probes with Fluorescein-Tagged Nucleotides
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    Chapter -1592590228 Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence
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    Chapter -1592590224 Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence
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    Chapter -1592590218 Hybridization and Competition Hybridization of Southern Blots
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    Chapter -1592590212 Autoradiography and Fluorography
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    Chapter -1592590204 Formaldehyde Gel Electrophoresis of Total RNA
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    Chapter -1592590200 RNA Probes for the Analysis of Gene Expression
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    Chapter -1592590186 Primer Extension Analysis of mRNA
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    Chapter -1592590180 S1 Mapping Using Single-Stranded DNA Probes
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    Chapter -1592590174 Measurements of Rate of Transcription in Isolated Nuclei by Nuclear “Run-Off” Assay
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    Chapter -1592590168 One-Tube RT-PCR with Sequence-Specific RT Primers
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    Chapter -1592590162 Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA
  37. Altmetric Badge
    Chapter -1592590152 Quantitative Analysis of RNA Species by Polymerase Chain Reaction and Solid-Phase Minisequencing
  38. Altmetric Badge
    Chapter -1592590142 Nonradioactive Northern Blotting of RNA
  39. Altmetric Badge
    Chapter -1592590132 Analysis of RNA by Northern Blotting Using Riboprobes
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    Chapter -1592590120 Production of Double-Stranded cDNA for Gene Library Synthesis
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    Chapter -1592590114 Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs
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    Chapter -1592590092 cDNA Library Construction Using Streptavidin-Paramagnetic Beads and PCR
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    Chapter -1592590086 Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products
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    Chapter -1592590076 Subtraction Hybridization cDNA Libraries
  45. Altmetric Badge
    Chapter -1592590062 Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
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    Chapter -1592590054 Extraction and Purification of Plasmid DNA
  47. Altmetric Badge
    Chapter -1592590048 Biotinylated Probes in Colony Hybridization
  48. Altmetric Badge
    Chapter -1592590042 Cloning Long Polymerase Chain Reaction Products
  49. Altmetric Badge
    Chapter -1592590034 Cloning DNA Fragments in M13 Vectors
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    Chapter -1592590026 cDNA Library Construction for the Lambda ZAP®-Based Vectors
  51. Altmetric Badge
    Chapter -1592590014 Expression and Preparation of Fusion Proteins from Recombinant λgt11 Phages
  52. Altmetric Badge
    Chapter -1592590008 Antibody Screening of Bacteriophage λgt11 DNA Expression Libraries
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    Chapter -1592590000 Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides
  54. Altmetric Badge
    Chapter -1592589992 cDNA Library Screening with the Tetramethylammonium Chloride (TM AC) Technique Using Highly Degenerate Oligonucleotide Probes
  55. Altmetric Badge
    Chapter -1592589984 Screening Recombinant Libraries by Polymerase Chain Reaction
  56. Altmetric Badge
    Chapter -1592589976 Construction and Screening of Cosmid Libraries
  57. Altmetric Badge
    Chapter -1592589966 Generation of Large Insert YAC Libraries
  58. Altmetric Badge
    Chapter -1592589956 YAC Library Storage and Transport
  59. Altmetric Badge
    Chapter -1592589950 YAC Library Screening
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    Chapter -1592589944 YAC Library Screening
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    Chapter -1592589932 Phage-Display Libraries of Murine and Human Antibody Fab Fragments
  62. Altmetric Badge
    Chapter -1592589900 Preparation and Analysis of DNA Sequencing Gels
  63. Altmetric Badge
    Chapter -1592589892 DNA Sequencing of Plasmids
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    Chapter -1592589888 Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors
  65. Altmetric Badge
    Chapter -1592589882 Direct cDNA Sequencing Using Sequential Linear/Asymmetric Polymerase Chain Reaction
  66. Altmetric Badge
    Chapter -1592589876 Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products
  67. Altmetric Badge
    Chapter -1592589866 Direct Polymerase Chain Reaction Sequencing with Denaturants
  68. Altmetric Badge
    Chapter -1592589858 Direct DNA Sequencing of Polymerase Chain Reaction Products Using Magnetic Beads
  69. Altmetric Badge
    Chapter -1592589848 Polymerase Chain Reaction Cycle Sequencing with Degenerate Primers
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    Chapter -1592589840 Direct Automated Cycle Sequencing of Polymerase Chain Reaction Products
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    Chapter -1592589834 Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
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    Chapter -1592589828 DNA Sequencing by the Chemical Method
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    Chapter -1592589824 One-Step One-Lane Chemical Sequencing of DNA
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    Chapter -1592589812 Polymerase Chain Reaction
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    Chapter -1592589800 Primer Selection and Design for Polymerase Chain Reaction
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    Chapter -1592589792 One-Step Optimization Using Touchdown and Stepdown Polymerase Chain Reaction
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    Chapter -1592589786 Cloning Gene Family Members Using Polymerase Chain Reaction with Degenerate Oligonucleotide Primers
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    Chapter -1592589772 Construction of Synthetic Genes by Polymerase Chain Reaction
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    Chapter -1592589768 Rapid Amplification of cDNA Ends
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    Chapter -1592589762 Multiplex Polymerase Chain Reaction
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    Chapter -1592589756 Inverse Polymerase Chain Reaction
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    Chapter -1592589748 Long-Range Polymerase Chain Reaction
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    Chapter -1592589736 Nonradioactive Differential Display of Messenger RNA
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    Chapter -1592589728 Gene Isolation by Exon Trapping
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    Chapter -1592589714 DNA Rescue by the Vectorette Method
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    Chapter -1592589706 Random Amplified Polymorphic DNA (RAPDs)
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    Chapter -1592589702 Restriction Fragment Length Polymorphism
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    Chapter -1592589696 Detection of Mutations in DNA and RNA by Chemical Cleavage
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    Chapter -1592589686 Mutation Screening Using PCR-SSCP
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    Chapter -1592589676 Detecting Point Mutations by Denaturing-Gradient Gel Electrophoresis
  91. Altmetric Badge
    Chapter -1592589664 Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
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    Chapter -1592589658 The Amplification Refractory Mutation System
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    Chapter -1592589652 DNase I Footprinting
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    Chapter -1592589644 Identification of Protein-DNA Contacts with Dimethyl Sulfate
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    Chapter -1592589636 The Gel Shift Assay for the Analysis of DNA-Protein Interactions
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    Chapter -1592589624 Yeast Two-Hybrid Library Screening
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    Chapter -1592589608 The Southwestern Assay
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    Chapter -1592589598 Nonradioactive Methods for the Detection of RNA-Protein Interaction
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    Chapter -1592589588 Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
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    Chapter -1592589574 Generating Nested Deletions with Exonuclease III
  101. Altmetric Badge
    Chapter -1592589566 Primer-Directed Site-Specific Mutagenesis
  102. Altmetric Badge
    Chapter -1592589554 Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template
  103. Altmetric Badge
    Chapter -1592589546 Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
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    Chapter -1592589536 Site-Directed Mutagenesis with LA-PCR™ Technology
  105. Altmetric Badge
    Chapter -1592589524 Recombination and Mutagenesis by Overlap Extension PCR
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    Chapter -1592589516 Site-Directed Mutagenesis and Gene Fusion by Megaprimer PCR
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    Chapter -1592589506 Transcription In Vitro Using Bacteriophage RNA Polymerases
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    Chapter -1592589496 In Vitro Translation of mRNA in a Rabbit Reticulocyte Lysate Cell-Free System
  109. Altmetric Badge
    Chapter -1592589490 In Vitro Translation of mRNA in a Wheat Germ Extract Cell-Free System
  110. Altmetric Badge
    Chapter -1592589486 The Xenopus Egg Extract Translation System
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    Chapter -1592589474 Manipulation of Baculovirus Vectors
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    Chapter -1592589460 Procedures for the Analysis and Purification of His-Tagged Proteins
  113. Altmetric Badge
    Chapter -1592589446 Detection and Immobilization of Proteins Containing the 6xHis Tag
  114. Altmetric Badge
    Chapter -1592589434 Expression and Purification of Recombinant Proteins Using the pET System
  115. Altmetric Badge
    Chapter -1592589400 Preparation of Tissue Sections and Slides for mRNA Hybridization
  116. Altmetric Badge
    Chapter -1592589396 Use of Digoxigenin-Labeled Probes on Tissue Sections
  117. Altmetric Badge
    Chapter -1592589390 Gene Mapping by FISH
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    Chapter -1592589370 Oligonucleotide PRINS DNA Synthesis
  119. Altmetric Badge
    Chapter -1592589364 Chromosome-Specific PRINS
  120. Altmetric Badge
    Chapter -1592589358 In Situ PCR Amplification of Intracellular mRNA
  121. Altmetric Badge
    Chapter -1592589350 An Introduction to Bioinformatics
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Title
The Nucleic Acid Protocols Handbook
Published by
Humana Press, Totowa, NJ, June 2008
DOI 10.1385/1592590381
ISBNs
978-0-89603-459-4, 978-1-59259-038-4
Authors

Ralph Rapley

Editors

Ralph Rapley

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Colombia 1 1%
Serbia 1 1%
Unknown 74 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 16 21%
Student > Ph. D. Student 15 19%
Researcher 13 17%
Student > Master 8 10%
Other 5 6%
Other 9 12%
Unknown 11 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 43%
Biochemistry, Genetics and Molecular Biology 18 23%
Medicine and Dentistry 3 4%
Physics and Astronomy 3 4%
Engineering 2 3%
Other 6 8%
Unknown 12 16%