| Title |
Scanning microprobe matrix-assisted laser desorption ionization (SMALDI) mass spectrometry: Instrumentation for sub-micrometer resolved LDI and MALDI surface analysis
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, June 2002
|
| DOI | 10.1016/s1044-0305(02)00376-8 |
| Pubmed ID | |
| Authors |
Bernhard Spengler, Martin Hubert |
| Abstract |
A new instrument and method is described for laterally resolved mass spectrometric surface analysis. Fields of application are in both the life sciences and the material sciences. The instrument provides for imaging of the distribution of selected sample components from natural and artificial surfaces. Samples are either analyzed by laser desorption ionization (LDI) time-of-flight mass spectrometry or, after preparation with a suitable matrix, by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. Areas of 100 x 100 microm are scanned with minimal increments of 0.25 microm, and between 10,000 and 160,000 mass spectra are acquired per image within 3 to 50 min (scan rate up to 50 pixels per s). The effective lateral resolution is in the range of 0.6 to 1.5 microm depending on sample properties, preparation methods and laser wavelength. Optical investigation of the same sample area by UV confocal scanning laser microscopy was found to be very attractive in combination with scanning MALDI mass analysis because pixel-identical images can be created with both techniques providing for a strong increase in analytical information. This article describes the method and instrumentation, including first applicational examples in elemental analysis, imaging of pine tree roots, and investigation of MALDI sample morphology in biomolecular analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 2% |
| United Kingdom | 1 | 1% |
| Unknown | 95 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 26 | 27% |
| Researcher | 25 | 26% |
| Student > Master | 9 | 9% |
| Student > Bachelor | 7 | 7% |
| Other | 5 | 5% |
| Other | 11 | 11% |
| Unknown | 15 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 36 | 37% |
| Biochemistry, Genetics and Molecular Biology | 8 | 8% |
| Agricultural and Biological Sciences | 8 | 8% |
| Physics and Astronomy | 6 | 6% |
| Engineering | 5 | 5% |
| Other | 16 | 16% |
| Unknown | 19 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2021.
All research outputs
#3,802,284
of 25,394,764 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#254
of 3,836 outputs
Outputs of similar age
#8,160
of 126,622 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#3
of 18 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,836 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 126,622 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.