| Title |
Biochemical properties of isoprene synthase in poplar (Populus × canescens)
|
|---|---|
| Published in |
Planta, July 2005
|
| DOI | 10.1007/s00425-005-0022-1 |
| Pubmed ID | |
| Authors |
J.-P. Schnitzler, I. Zimmer, A. Bachl, M. Arend, J. Fromm, R. J. Fischbach |
| Abstract |
Isoprene synthase (ISPS) catalyzes the elimination of pyrophosphate from dimethylallyl diphosphate (DMADP) forming isoprene, a volatile hydrocarbon emitted from many plant species to the atmosphere. In the present work, immunological techniques were applied to study and localize ISPS in poplar leaves (Populus x canescens). Immunogold labeling using polyclonal antibodies generated against His-tagged recombinant ISPS protein detected ca. 44% of ISPS in the stroma of the chloroplasts and ca. 56% of gold particles attached to the stromal-facing side of the thylakoid membranes. ISPS isolated from leaves exhibited the same biochemical properties as the recombinant ISPS without the plastid-targeting peptide heterologous expressed in E. coli, whereas an additional C- or N-terminal His-tag changed the biochemical features of the recombinant enzyme with regard to temperature, pH, and substrate dependence. In comparison to the closely related class of monoterpene synthases from angiosperms and ISPS of oaks, the most striking feature of the poplar ISPS is a cooperative substrate dependence which is characteristic to enzymes with positive substrate activation. The detection of four immunoreactive bands in poplar leaf extracts with isoelectric points from 5.0 to 5.5 and a native molecular weight of ca. 51 kDa give reason for future studies on post-translational modifications of ISPS. |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 1% |
| Germany | 1 | 1% |
| Unknown | 67 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 23% |
| Researcher | 14 | 20% |
| Student > Bachelor | 7 | 10% |
| Professor > Associate Professor | 6 | 9% |
| Student > Master | 5 | 7% |
| Other | 11 | 16% |
| Unknown | 10 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 27 | 39% |
| Biochemistry, Genetics and Molecular Biology | 9 | 13% |
| Environmental Science | 5 | 7% |
| Engineering | 4 | 6% |
| Chemistry | 4 | 6% |
| Other | 6 | 9% |
| Unknown | 14 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 September 2020.
All research outputs
#3,919,343
of 26,017,215 outputs
Outputs from Planta
#150
of 3,151 outputs
Outputs of similar age
#8,001
of 71,676 outputs
Outputs of similar age from Planta
#1
of 31 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,151 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 71,676 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.