| Title |
Molecular evidence for human alpha2-HS glycoprotein (AHSG) polymorphism
|
|---|---|
| Published in |
Human Genetics, December 1996
|
| DOI | 10.1007/s004390050302 |
| Pubmed ID | |
| Authors |
M. Osawa, Kazuo Umetsu, Tamotsu Ohki, Toshio Nagasawa, Tsuneo Suzuki, Sanae Takeichi |
| Abstract |
Alpha 2-HS glycoprotein (AHSG) is a human plasma glycoprotein that exhibits genetic polymorphism on isoelectric focusing (IEF). To identify the origin of two common alleles, AHSG*1 and *2, we examined nucleotide exchanges in the gene. AHSG cDNA was obtained by RT-PCR from poly(A) RNA of seven liver tissue samples and subcloned into a plasmid vector. After sequencing, we found six single nucleotide differences in comparison with the originally reported sequence. In particular, the nucleotide substitutions of C to T at amino acid position 230 and C to G at position 238 were common among the samples exhibiting phenotype 2-1 or 2. Since these substitutions might give rise to a NlaIII site and a SacI site, respectively, for the potential AHSG*2, we analyzed these substitutions by PCR-RFLP using genomic DNA of 68 individuals. The result was consistent with the IEF analysis of the corresponding serum, indicating that AHSG*1 was characterized by ACG (Thr) at position 230 in exon 6 and ACC (Thr) at position 238 in exon 7, and that AHSG*2 was characterized by ATG (Met) at position 230 and AGC (Ser) at position 238. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 20% |
| Unknown | 4 | 80% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 20% |
| Student > Ph. D. Student | 1 | 20% |
| Student > Master | 1 | 20% |
| Researcher | 1 | 20% |
| Professor > Associate Professor | 1 | 20% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 1 | 20% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Chemistry | 1 | 20% |
| Medicine and Dentistry | 1 | 20% |
| Unknown | 1 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 December 2007.
All research outputs
#8,533,995
of 25,371,288 outputs
Outputs from Human Genetics
#1,014
of 2,957 outputs
Outputs of similar age
#19,846
of 92,554 outputs
Outputs of similar age from Human Genetics
#6
of 24 outputs
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