| Title |
Yeast Srp1, a nuclear protein related toDrosophila and mouse pendulin, is required for normal migration, division, and integrity of nuclei during mitosis
|
|---|---|
| Published in |
Molecular Genetics and Genomics, August 1995
|
| DOI | 10.1007/bf02191602 |
| Pubmed ID | |
| Authors |
Polonca Küssel, Manfred Frasch |
| Abstract |
This paper describes genes from yeast and mouse with significant sequence similarities to a Drosophila gene that encodes the blood cell tumor suppressor pendulin. The protein encoded by the yeast gene, Srp1p, and mouse pendulin share 42% and 51% amino acid identity with Drosophila pendulin, respectively. All three proteins consist of 10.5 degenerate tandem repeats of approximately 42 amino acids each. Similar repeats occur in a superfamily of proteins that includes the Drosophila Armadillo protein. All three proteins contain a consensus sequence for a bipartite nuclear localization signal (NLS) in the N-terminal domain, which is not part of the repeat structure. Confocal microscopic analysis of yeast cells stained with antibodies against Srp1p reveals that this protein is intranuclear throughout the cell cycle. Targeted gene disruption shows that SRP1 is an essential gene. Despite their sequence similarities, Drosophila and mouse pendulin are unable to rescue the lethality of an SRP1 disruption. We demonstrate that yeast cells depleted of Srp1p arrest in mitosis with a G2 content of DNA. Arrested cells display abnormal structures and orientations of the mitotic spindles, aberrant segregation of the chromatin and the nuclei, and threads of chromatin emanating from the bulk of nuclear DNA. This phenotype suggests that Srp1p is required for the normal function of microtubules and the spindle pole bodies, as well as for nuclear integrity. We suggest that Srp1p interacts with multiple components of the cell nucleus that are required for mitosis and discuss its functional similarities to, and differences from Drosophila pendulin. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 7% |
| Egypt | 1 | 7% |
| Unknown | 13 | 87% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 27% |
| Student > Master | 3 | 20% |
| Professor | 3 | 20% |
| Researcher | 3 | 20% |
| Student > Bachelor | 1 | 7% |
| Other | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 73% |
| Veterinary Science and Veterinary Medicine | 2 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Environmental Science | 1 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 July 2019.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from Molecular Genetics and Genomics
#920
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Outputs of similar age
#7,016
of 22,271 outputs
Outputs of similar age from Molecular Genetics and Genomics
#21
of 49 outputs
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