| Title |
Effects of temperature on human L-type cardiac Ca2+ channels expressed in Xenopus oocytes
|
|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, May 1998
|
| DOI | 10.1007/s004240050628 |
| Pubmed ID | |
| Authors |
T. J. A. Allen, Gabor Mikala |
| Abstract |
Temperature normally affects peak L-type Ca2+ channel (CaCh) current with a temperature coefficient (Q10) of between 1.8 and 3.5; in cardiomyocytes attenuating protein kinase A activity increases Q10 whilst activating it lowers Q10. We examine temperature effects using cloned human cardiac CaChs expressed in Xenopus oocytes. Peak inward currents (IBa) through expressed CaChs (i.e. alpha1C alpha2/deltaa beta1b) exhibited a Q10 of 5.8+/-0.4 when examined between 15 and 25 degreesC. The nifedipine-sensitive IBa exhibited a higher Q10 of 8.7+/-0.5, whilst the nifedipine-insensitive IBa exhibited Q10 of 3.7+/-0.3. Current/voltage (I/V) relationships shifted to negative potentials on warming. Using instead a different CaCh beta subunit isoform, beta2c, gave rise to an IBa similar to those expressed using beta1b. We utilized a carboxyl deletion mutant, alpha1C-Delta1633, to determine the temperature sensitivity of the pore moiety in the absence of auxiliary subunits; IBa through this channel exhibited a Q10 of 9.3+/-0.3. However, the Q10 for macroscopic conductance was reduced compared to that of heteromeric channels; decreasing from 5.0 (i.e. alpha1C alpha2/deltaa beta1b) and 3.9 (i.e. alpha1C alpha2/deltaa beta2c) to 2.4 (alpha1C-Delta1633). These observations differ markedly from those made in studies of cardiomyocytes, and suggest that enhanced sensitivity may depend on the membrane environment, channel assembly or other regulatory factors. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 20% |
| United States | 1 | 10% |
| Unknown | 7 | 70% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Professor | 2 | 20% |
| Researcher | 2 | 20% |
| Student > Bachelor | 1 | 10% |
| Student > Postgraduate | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 50% |
| Neuroscience | 2 | 20% |
| Biochemistry, Genetics and Molecular Biology | 1 | 10% |
| Unknown | 2 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2007.
All research outputs
#8,533,995
of 25,371,288 outputs
Outputs from Pflügers Archiv - European Journal of Physiology
#513
of 2,055 outputs
Outputs of similar age
#10,691
of 33,408 outputs
Outputs of similar age from Pflügers Archiv - European Journal of Physiology
#1
of 5 outputs
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