| Title |
Tissue distribution and functional expression of the human voltage-gated sodium channel β3 subunit
|
|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, January 2001
|
| DOI | 10.1007/s004240000449 |
| Pubmed ID | |
| Authors |
Edward B. Stevens, Peter J. Cox, Bhaval S. Shah, Alistair K. Dixon, Peter J. Richardson, Robert D. Pinnock, Kevin Lee |
| Abstract |
This study investigated the distribution of beta3 in human tissues and the functional effects of the human beta3 subunit on the gating properties of brain and skeletal muscle alpha subunits. Using RT-PCR of human cDNA panels, beta3 message was detected in brain, heart, kidney, lung, pancreas and skeletal muscle. Both alphaIIA and SkM1 expressed in Xenopus oocytes inactivated with a time course described by two exponential components representing fast and slow gating modes, while co-expression of human beta3 with alphaIIA or SkM1 significantly increased the proportion of channels operating by the fast gating mode. In the presence of beta3 a greater proportion of alphaIIA or SkM1 current was described by the fast time constant for both inactivation and recovery from inactivation. beta3 caused a hyperpolarizing shift in the voltage dependence of inactivation of alphaIIIA and reduced the slope factor. The voltage dependence of inactivation of SkM1 was described by a double Boltzmann equation. However, SkM1 co-expressed with beta3 was described by a single Boltzmann equation similar to one of the Boltzmann components for SkM1 expressed alone, with a small positive shift in V1/2 value and reduced slope factor. This is the first study demonstrating that beta3 is expressed in adult mammalian skeletal muscle and can functionally couple to the skeletal muscle alpha subunit, SkM1. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 46% |
| Other | 3 | 23% |
| Student > Ph. D. Student | 1 | 8% |
| Professor | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 46% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 15% |
| Neuroscience | 2 | 15% |
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 1 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 December 2007.
All research outputs
#8,534,976
of 25,373,627 outputs
Outputs from Pflügers Archiv - European Journal of Physiology
#513
of 2,055 outputs
Outputs of similar age
#26,247
of 114,345 outputs
Outputs of similar age from Pflügers Archiv - European Journal of Physiology
#2
of 9 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,055 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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