| Title |
Claudin-17 forms tight junction channels with distinct anion selectivity
|
|---|---|
| Published in |
Cellular and Molecular Life Sciences, March 2012
|
| DOI | 10.1007/s00018-012-0949-x |
| Pubmed ID | |
| Authors |
Susanne M. Krug, Dorothee Günzel, Marcel P. Conrad, Rita Rosenthal, Anja Fromm, Salah Amasheh, Jörg D. Schulzke, Michael Fromm |
| Abstract |
Barrier properties of tight junctions are determined by the claudin protein family. Many claudins seal this barrier, but others form paracellular channels. Among these, no claudins with general and clear-cut anion selectivity have yet been described, while for claudin-10a and claudin-4, only circumstantial or small anion selectivities have been shown. A claudin with unknown function and tissue distribution is claudin-17. We characterized claudin-17 by overexpression and knock-down in two renal cell lines. Overexpression in MDCK C7 cell layers caused a threefold increase in paracellular anion permeability and switched these cells from cation- to anion-selective. Knockdown in LLC-PK(1) cells indorsed the finding of claudin-17-based anion channels. Mutagenesis revealed that claudin-17 anion selectivity critically depends on a positive charge at position 65. Claudin-17 expression was found in two organs: marginal in brain but abundant in kidney, where expression was intense in proximal tubules and gradually decreased towards distal segments. As claudin-17 is predominantly expressed in proximal nephrons, which exhibit substantial, though molecularly not defined, paracellular chloride reabsorption, we suggest that claudin-17 has a unique physiological function in this process. In conclusion, claudin-17 forms channels within tight junctions with distinct anion preference. |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| France | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 46 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 27% |
| Student > Ph. D. Student | 8 | 16% |
| Student > Doctoral Student | 7 | 14% |
| Student > Bachelor | 7 | 14% |
| Professor | 4 | 8% |
| Other | 6 | 12% |
| Unknown | 4 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 35% |
| Agricultural and Biological Sciences | 13 | 27% |
| Medicine and Dentistry | 8 | 16% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Linguistics | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 6 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#7,845,540
of 23,794,258 outputs
Outputs from Cellular and Molecular Life Sciences
#1,655
of 4,151 outputs
Outputs of similar age
#52,720
of 158,447 outputs
Outputs of similar age from Cellular and Molecular Life Sciences
#12
of 34 outputs
Altmetric has tracked 23,794,258 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
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