| Title |
Probiotic Potential of Lactobacillus Strains Isolated from Sorghum-Based Traditional Fermented Food
|
|---|---|
| Published in |
Probiotics and Antimicrobial Proteins, February 2015
|
| DOI | 10.1007/s12602-015-9186-6 |
| Pubmed ID | |
| Authors |
K. Poornachandra Rao, G. Chennappa, U. Suraj, H. Nagaraja, A. P. Charith Raj, M. Y. Sreenivasa |
| Abstract |
Sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. The isolates were identified by biochemical, physiological and genetic methods. Species identification was done by 16s rRNA sequence analysis. The functional probiotic potential of the two Lactobacillus species viz., Lactobacillus plantarum (Lact. plantarum) and Lactobacillus pentosus (Lact. pentosus) was assessed by different standard parameters. The strains were tolerant to pH 2 for 1 h and resistant to methicillin, kanamycin, vancomycin and norfloxacin. Two (Lact. plantarum COORG-3 and Lact. pentosus COORG-8) out of eight isolates recorded the cell surface hydrophobicity to be 59.12 and 64.06 %, respectively. All the strains showed tolerance to artificial duodenum juice (pH 2) for 3 h, positive for bile salt hydrolase test and negative for haemolytic test. The neutralized cell-free supernatant of the strains Lact. pentosus COORG-4, Lact. plantarum COORG-1, Lact. plantarum COORG-7, Lact. pentosus COORG-8 and Lact. plantarum COORG-3 showed good antibiofilm activity. Lact. pentosus COORG-8 exhibited 74 % activity against Pseudomonas aeruginosa-MTCC 7903 and Lact. plantarum COORG-7 showed 68 % inhibition of biofilm against Klebsiella pneumonia MTCC 7407. Three (Lact. plantarum COORG-7, Lact. pentosus COORG-5 and Lact. pentosus COORG 8) out of eight isolates exhibited a good antimicrobial activity against Listeria monocytogenes and five isolates (Lact. pentosus COORG 2, Lact. plantarum COORG 1, Lact. plantarum COORG 4, Lact. pentosus COORG 3 and Lact. plantarum COORG 6) are active against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumonia, Enterococcus faecalis. The study also evaluated the cholesterol lowering property of the Lactobacillus strains using hen egg yolk as the cholesterol source. The cholesterol in hen egg yolk was assimilated by 74.12 and 68.26 % by Lact. plantarum COORG 4 and Lact. pentosus COORG 7, respectively. The results of the present study suggest that the Lactobacillus strains isolated and characterized from sorghum-based fermented product may be used as probiotic strains for therapeutic applications. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 131 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 131 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 16% |
| Student > Master | 21 | 16% |
| Student > Bachelor | 18 | 14% |
| Researcher | 17 | 13% |
| Other | 5 | 4% |
| Other | 22 | 17% |
| Unknown | 27 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 42 | 32% |
| Immunology and Microbiology | 21 | 16% |
| Biochemistry, Genetics and Molecular Biology | 12 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 3% |
| Nursing and Health Professions | 3 | 2% |
| Other | 16 | 12% |
| Unknown | 33 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 August 2016.
All research outputs
#13,751,991
of 23,314,015 outputs
Outputs from Probiotics and Antimicrobial Proteins
#180
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Outputs of similar age
#179,438
of 359,963 outputs
Outputs of similar age from Probiotics and Antimicrobial Proteins
#3
of 8 outputs
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So far Altmetric has tracked 557 research outputs from this source. They receive a mean Attention Score of 3.8. This one has gotten more attention than average, scoring higher than 66% of its peers.
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