| Title |
Design and application of antisense oligonucleotides in cell culture,in vivo, and as therapeutic agents
|
|---|---|
| Published in |
Cellular and Molecular Neurobiology, October 1994
|
| DOI | 10.1007/bf02088837 |
| Pubmed ID | |
| Authors |
Wolfgang Brysch, Karl-Hermann Schlingensiepen |
| Abstract |
1. Synthetic oligonucleotides can inhibit the expression of a gene in a sequence specific manner on the transcriptional and translational level. These molecules are usually referred to as antisense oligonucleotides. 2. Antisense mediated inhibition of gene expression is a valuable tool to analyze the function of a gene in vivo and can also be used for therapeutic gene suppression. 3. A number of factors such as the mode of action, specificity, chemistry, and pharmacology must be carefully considered for the design and successful application of antisense oligonucleotides. 4. Assay systems and controls must be chosen as to assure that the observed biological effects of antisense oligonucleotides do in fact reflect the result of a specific gene inhibition. 5. This article critically discusses these factors in view of the literature and our own experience with a wide range of cell types and animal models, targeting different genes. The emphasis is on the use of phosphorothioate oligodeoxynucleotides in cell cultures, in vivo, and as potential drugs. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 38% |
| Student > Ph. D. Student | 4 | 25% |
| Professor | 3 | 19% |
| Other | 1 | 6% |
| Professor > Associate Professor | 1 | 6% |
| Other | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 8 | 50% |
| Agricultural and Biological Sciences | 5 | 31% |
| Medicine and Dentistry | 3 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2017.
All research outputs
#5,016,753
of 23,854,458 outputs
Outputs from Cellular and Molecular Neurobiology
#237
of 1,046 outputs
Outputs of similar age
#2,834
of 22,644 outputs
Outputs of similar age from Cellular and Molecular Neurobiology
#1
of 8 outputs
Altmetric has tracked 23,854,458 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,046 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one has gotten more attention than average, scoring higher than 74% of its peers.
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We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them