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The activation of human gene MAGE-1 in tumor cells is correlated with genome-wide demethylation.

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, July 1996
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

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32 patents
wikipedia
2 Wikipedia pages

Citations

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439 Dimensions

Readers on

mendeley
114 Mendeley
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1 CiteULike
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Title
The activation of human gene MAGE-1 in tumor cells is correlated with genome-wide demethylation.
Published in
Proceedings of the National Academy of Sciences of the United States of America, July 1996
DOI 10.1073/pnas.93.14.7149
Pubmed ID
Authors

C De Smet, O De Backer, I Faraoni, C Lurquin, F Brasseur, T Boon

Abstract

Human gene MAGE-1 encodes tumor-specific antigens that are recognized on melanoma cells by autologous cytolytic T lymphocytes. This gene is expressed in a significant proportion of tumors of various histological types, but not in normal tissues except male germ-line cells. We reported previously that reporter genes driven by the MAGE-1 promoter are active not only in the tumor cell lines that express MAGE-1 but also in those that do not. This suggests that the critical factor causing the activation of MAGE-1 in certain tumors is not the presence of the appropriate transcription factors. The two major MAGE-1 promoter elements have an Ets binding site, which contains a CpG dinucleotide. We report here that these CpG are demethylated in the tumor cell lines that express MAGE-1, and are methylated in those that do not express the gene. Methylation of these CpG inhibits the binding of transcription factors, as seen by mobility shift assay. Treatment with the demethylating agent 5-aza-2'-deoxycytidine activated gene MAGE-1 not only in tumor cell lines but also in primary fibroblasts. Finally, the overall level of CpG methylation was evaluated in 20 different tumor cell lines. It was inversely correlated with the expression of MAGE-1. We conclude that the activation of MAGE-1 in cancer cells is due to the demethylation of the promoter. This appears to be a consequence of a genome-wide demethylation process that occurs in many cancers and is correlated with tumor progression.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 114 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 <1%
Unknown 113 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 21%
Researcher 21 18%
Student > Master 20 18%
Student > Bachelor 8 7%
Student > Doctoral Student 6 5%
Other 17 15%
Unknown 18 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 28 25%
Agricultural and Biological Sciences 27 24%
Medicine and Dentistry 21 18%
Immunology and Microbiology 5 4%
Chemistry 3 3%
Other 5 4%
Unknown 25 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 October 2023.
All research outputs
#2,232,164
of 24,622,191 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#26,482
of 101,438 outputs
Outputs of similar age
#861
of 30,245 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#23
of 474 outputs
Altmetric has tracked 24,622,191 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 101,438 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 38.8. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 30,245 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 474 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.