| Title |
The human vigilin gene: identification, chromosomal localization and expression pattern
|
|---|---|
| Published in |
Human Genetics, May 1994
|
| DOI | 10.1007/bf00202827 |
| Pubmed ID | |
| Authors |
Gabriele Plenz, Sebastian Kügler, Susanne Schnittger, Harald Rieder, Christa Fonatsch, Peter K. Müller |
| Abstract |
Chick vigilin cRNA clones were used to isolate the cognate human gene, by screening a pWE15 genomic library. Three independent cosmid clones were isolated and characterized by restriction mapping. The gene was identified by sequencing an internal EcoRI fragment containing two exons homologous to exon 24 and 25 of the chicken vigilin gene and corresponding to nucleotides 1973-2104 of the human HBP-cDNA. The homology between the chicken and human sequences was 77% and 82% at the cDNA level, and 91% and 100% at the amino acid level. In addition, the analyzed intron/exon boundaries were invariantly conserved. The 5' and 3' regions of the human gene were mapped by Southern analysis of the respective clones with synthetic oligonucleotides. The entire vigilin gene spans a region of about 50 kb and has been assigned to chromosome 2q36-q37.2 (FL-pter value of 0.96 +/- 0.03) by fluorescence in situ hybridization to metaphase spreads from normal peripheral blood lymphocytes. The vigilin gene is localized in a chromosomal region comprising a cluster of collagen genes (COLIVA3, COLVIA3) and the locus of the Waardenburg syndrome I. Only one mRNA species of 4.4 kb is transcribed from the human vigilin gene. In accordance with previous observations on chicken mRNA, the expression of the human vigilin mRNA depends on the stage of cytodifferentiation both in vitro and in situ. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 9% |
| Israel | 1 | 9% |
| Germany | 1 | 9% |
| Unknown | 8 | 73% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 36% |
| Student > Ph. D. Student | 3 | 27% |
| Student > Bachelor | 1 | 9% |
| Student > Master | 1 | 9% |
| Student > Doctoral Student | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 55% |
| Immunology and Microbiology | 2 | 18% |
| Biochemistry, Genetics and Molecular Biology | 1 | 9% |
| Unknown | 2 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#7,454,298
of 22,789,076 outputs
Outputs from Human Genetics
#933
of 2,953 outputs
Outputs of similar age
#6,554
of 22,556 outputs
Outputs of similar age from Human Genetics
#2
of 14 outputs
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