| Title |
Cloning, sequencing and heterologous expression of the monoamine oxidase gene from Aspergillus niger
|
|---|---|
| Published in |
Molecular Genetics and Genomics, July 1995
|
| DOI | 10.1007/bf00293144 |
| Pubmed ID | |
| Authors |
Boris Schilling, Konrad Lerch |
| Abstract |
The gene encoding the flavin-containing monoamine oxidase (MAO-N) of the filamentous fungus Aspergillus niger was cloned. MAO-N is the first nonvertebrate monoamine oxidase described to date. Three partial cDNA clones, isolated from an expression library, were used to identify and clone the structural gene (maoN) from an A. niger genomic DNA library. The maoN gene was sequenced, and analysis revealed an open reading frame that codes for a protein of 495 amino acids with a calculated molecular mass of 55.6 kDa. Sequencing of an internal proteolytic fragment of the purified enzyme confirmed the derived amino acid sequence. Analysis of the deduced amino acid sequence indicates that MAO-N is structurally related to the human monoamine oxidases MAO-A and MAO-B. In particular, the regions known to be involved in the binding of the FAD cofactor show a high degree of homology; however, the conserved cysteine residue to which the flavin cofactor is covalently bound in the mammalian forms is absent in the fungal enzyme. MAO-N has the C-terminal tripeptide Ala-Arg-Leu, which corresponds to the consensus targeting sequence found in many peroxisomal enzymes. The full-length cDNA for MAO-N was expressed in Escherichia coli from the T7 promoter of the expression vector pET3a, yielding a soluble and fully active enzyme form. |
Mendeley readers
The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Greece | 1 | 2% |
| Germany | 1 | 2% |
| Switzerland | 1 | 2% |
| Unknown | 55 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 29% |
| Researcher | 12 | 20% |
| Student > Master | 10 | 17% |
| Professor | 5 | 8% |
| Professor > Associate Professor | 3 | 5% |
| Other | 4 | 7% |
| Unknown | 8 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 19 | 32% |
| Chemistry | 11 | 19% |
| Biochemistry, Genetics and Molecular Biology | 10 | 17% |
| Engineering | 4 | 7% |
| Veterinary Science and Veterinary Medicine | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 12 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2019.
All research outputs
#2,863,833
of 25,373,627 outputs
Outputs from Molecular Genetics and Genomics
#44
of 3,318 outputs
Outputs of similar age
#987
of 22,673 outputs
Outputs of similar age from Molecular Genetics and Genomics
#1
of 33 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,318 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 97% of its peers.
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We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.