| Title |
Amorpha-4,11-diene synthase: cloning and functional expression of a key enzyme in the biosynthetic pathway of the novel antimalarial drug artemisinin
|
|---|---|
| Published in |
Planta, February 2001
|
| DOI | 10.1007/s004250000428 |
| Pubmed ID | |
| Authors |
T. Eelco Wallaart, Harro J. Bouwmeester, Jacques Hille, Lucas Poppinga, Niels C. A. Maijers |
| Abstract |
The sesquiterpenoid artemisinin, isolated these from the plant Artemisia annua L., and its semi-synthetic derivatives are a new and very effective group of antimalarial drugs. A branch point in the biosynthesis of this compound is the cyclisation of the ubiquitous precursor farnesyl diphosphate into the first specific precursor of artemisinin, namely amorpha-4,11-diene. Here we describe the isolation of a cDNA clone encoding amorpha-4,11-diene synthase. The deduced amino acid sequence exhibits the highest identity (50%) with a putative sesquiterpene cyclase of A. annua. When expressed in Escherichia coli, the recombinant enzyme catalyses the formation of amorpha-4,11-diene from farnesyl diphosphate. Introduction of the gene into tobacco (Nicotiana tabacum L.) resulted in the expression of an active enzyme and the accumulation of amorpha-4,11-diene ranging from 0.2 to 1.7 ng per g fresh weight. |
Mendeley readers
The data shown below were compiled from readership statistics for 99 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 1% |
| Brazil | 1 | 1% |
| Thailand | 1 | 1% |
| United States | 1 | 1% |
| Serbia | 1 | 1% |
| Unknown | 94 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 27 | 27% |
| Researcher | 18 | 18% |
| Student > Master | 14 | 14% |
| Student > Doctoral Student | 6 | 6% |
| Professor | 5 | 5% |
| Other | 17 | 17% |
| Unknown | 12 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 51 | 52% |
| Chemistry | 12 | 12% |
| Biochemistry, Genetics and Molecular Biology | 11 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 5% |
| Engineering | 4 | 4% |
| Other | 5 | 5% |
| Unknown | 11 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 May 2013.
All research outputs
#3,798,287
of 25,373,627 outputs
Outputs from Planta
#142
of 2,980 outputs
Outputs of similar age
#6,838
of 113,954 outputs
Outputs of similar age from Planta
#1
of 16 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,980 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 113,954 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.