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The Protein Protocols Handbook

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Cover of 'The Protein Protocols Handbook'

Table of Contents

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    Book Overview
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    Chapter 1 Protein Determination by UV Absorption
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    Chapter 2 The Lowry Method for Protein Quantitation
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    Chapter 3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
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    Chapter 4 The Bradford Method For Protein Quantitation
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    Chapter 5 Ultrafast Protein Determinations Using Microwave Enhancement
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    Chapter 6 The Nitric Acid Method for Protein Estimation in Biological Samples
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    Chapter 7 Quantitation of Tryptophan in Proteins
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    Chapter 8 Kinetic Silver Staining of Proteins
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    Chapter 9 Quantitation of Cellular Proteins by Flow Cytometry
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    Chapter 10 Quantitation of Cellular Proteins by Laser Scanning Cytometry
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    Chapter 11 Protein Solubility in Two-Dimensional Electrophoresis
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    Chapter 12 Mouse and Human Tissue Sample Preparation for 2-D Electrophoresis
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    Chapter 13 Plant Protein Sample Preparation for 2-DE
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    Chapter 14 Preparation of Bacterial Samples for 2-D PAGE
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    Chapter 15 Preparation of Bodily Fluids for 2-D PAGE
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    Chapter 16 Immunoaffinity Depletion of High Abundance Plasma and Serum Proteins
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    Chapter 17 Preparation of Yeast Samples for 2-D PAGE
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    Chapter 18 Membrane Protein Preparation Using Aqueous Polymer Two-Phase Systems
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    Chapter 19 Subcellular Fractionation of Small Sample Amounts
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    Chapter 20 Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins
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    Chapter 21 SDS Polyacrylamide Gel Electrophoresis of Proteins
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    Chapter 22 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins
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    Chapter 23 SDS-Polyacrylamide Gel Electrophoresis of Peptides
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    Chapter 24 Separation of Proteins by Blue Native Electrophoresis (BN-PAGE)
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    Chapter 25 Separation of Proteins by Gel Electrophoresis in the Tris-Taurine-HCl System
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    Chapter 26 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins
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    Chapter 27 Acetic Acid—Urea Polyacrylamide Gel Electrophoresis of Basic Proteins
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    Chapter 28 Acid—Urea—Triton Polyacrylamide Gel Electrophoresis of Histones
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    Chapter 29 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels
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    Chapter 30 Serial Immobilized pH Gradient Isoelectric Focusing over pH 4–9
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    Chapter 31 Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-Dimensional Electrophoresis
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    Chapter 32 Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins Using Carrier Ampholyte pH Gradients in the First Dimension
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    Chapter 33 The Protein Protocols Handbook
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    Chapter 34 Two-Dimensional PAGE of High-Molecular-Mass Proteins
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    Chapter 35 Casting Immobilized pH Gradients
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    Chapter 36 A Modified Nonequilibrium pH Gradient Electrophoresis (NEPHGE) Technique for Protein Separation
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    Chapter 37 Microchip Capillary Electrophoresis
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    Chapter 38 Protein Separations in Microfluidic Chips
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    Chapter 39 Difference Gel Electrophoresis (DIGE)
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    Chapter 40 Comparing 2D Electrophoretic Gels Across Internet Databases: An Open Source Application
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    Chapter 41 Quantification of Radiolabeled Proteins in Polyacrylamide Gels
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    Chapter 42 Differential ProteoTope Radioactive Quantification of Protein Abundance Ratios
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    Chapter 43 Quantification of Proteins on Polyacrylamide Gels
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    Chapter 44 Using SDS-PAGE and Scanning Laser Densitometry to Measure Yield and Degradation of Proteins
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    Chapter 45 Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red
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    Chapter 46 Zn 2+ -Reverse Staining Technique
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    Chapter 47 Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels
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    Chapter 48 Detection of Proteins in Polyacrylamide Gels by Silver Staining
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    Chapter 49 Background-Free Protein Detection in Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains
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    Chapter 50 Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining
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    Chapter 51 Detection of Glycoproteins in Gels and Blots
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    Chapter 52 Staining of Glycoproteins/Proteoglycans on SDS-Gels
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    Chapter 53 Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain
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    Chapter 54 A Modified Pro-Q Diamond Staining Protocol for Phosphoprotein Detection in Polyacrylamide Gels
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    Chapter 55 Electroelution of Proteins from Polyacrylamide Gels
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    Chapter 56 Autoradiography and Fluorography of Acrylamide Gels
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    Chapter 57 Proteolytic Activity Detection by Two-Dimensional Zymography
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    Chapter 58 Protein Blotting by Electroblotting
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    Chapter 59 Protein Blotting by the Semidry Method
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    Chapter 60 Protein Blotting by the Capillary Method
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    Chapter 61 Western Blotting of Basic Proteins Electrophoretically Resolved on Acid-Urea-Triton-Polyacrylamide Gels
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    Chapter 62 Immunoblotting of 2-DE Separated Proteins
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    Chapter 63 High-Efficiency Blotting of High-Molecular Weight Proteins
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    Chapter 64 Alkaline Phosphatase Labeling of IgG Antibody
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    Chapter 65 β-Galactosidase Labeling of IgG Antibody
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    Chapter 66 Horseradish Peroxidase Labeling of IgG Antibody
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    Chapter 67 Digoxigenin Labeling of IgG Antibody
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    Chapter 68 Conjugation of Fluorochromes to Antibodies
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    Chapter 69 Coupling of Antibodies with Biotin
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    Chapter 70 Preparation of Avidin Conjugates
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    Chapter 71 MDPF Staining of Proteins on Western Blots
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    Chapter 72 Copper Iodide Staining of Proteins and Its Silver Enhancement
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    Chapter 73 Detection of Proteins on Blots Using Direct Blue 71
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    Chapter 74 Detection of Proteins on Western Blots Using Colorimetric and Radiometric Visualization of Secondary Ligands
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    Chapter 75 Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting
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    Chapter 76 A Sensitive Method to Quantitatively Detect Total Protein on Membranes after Electrophoretic Transfer Using Avidin- or Streptavidin-Biotin
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    Chapter 77 Detection and Quantification of Proteins on Immunoblots using Enhanced Chemiluminescence
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    Chapter 78 Reutilization of Western Blots After Chemiluminescent or Autoradiographic Detection
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    Chapter 79 The Use of Quantum Dot Luminescent Probes for Western Blot Analysis
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    Chapter 80 The Use of Infrared Fluorescent Dyes in Quantitative Immunoblotting
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    Chapter 81 The Use of Infrared Fluorescent Dyes in Immunofluorescence Microscopy
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    Chapter 82 Carboxymethylation of Cysteine Using lodoacetamide/lodoacetic Acid
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    Chapter 83 Performic Acid Oxidation
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    Chapter 84 Succinylation of Proteins
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    Chapter 85 Pyridylethylation of Cysteine Residues
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    Chapter 86 Side Chain Selective Chemical Modifications of Proteins
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    Chapter 87 Nitration of Tyrosines
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    Chapter 88 Ethoxyformylation of Histidine
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    Chapter 89 Modification of Arginine Side Chains with p -Hydroxyphenylglyoxal
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    Chapter 90 Amidation of Carboxyl Groups
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    Chapter 91 Amidination of Lysine Side Chains
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    Chapter 92 Modification of Tryptophan with 2-Hydroxy-5-Nitrobenzylbromide
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    Chapter 93 Modification of Sulfhydryl Groups with DTNB
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    Chapter 94 Chemical Cleavage of Proteins at Methionyl-X Peptide Bonds
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    Chapter 95 Chemical Cleavage of Proteins at Tryptophanyl-X Peptide Bonds
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    Chapter 96 Chemical Cleavage of Proteins at Aspartyl-X Peptide Bonds
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    Chapter 97 Chemical Cleavage of Proteins at Cysteinyl-X Peptide Bonds
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    Chapter 98 Chemical Cleavage of Proteins at Asparaginyl-Glycyl Peptide Bonds
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    Chapter 99 Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
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    Chapter 100 On-PVDF Protein Digestions for N-terminal Sequencing and Peptide Mass Fingerprinting
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    Chapter 101 Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis
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    Chapter 102 Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
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    Chapter 103 Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography
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    Chapter 104 Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
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    Chapter 105 Peptide Mapping for Protein Characterization
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    Chapter 106 Production of Protein Hydrolysates Using Enzymes
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    Chapter 107 Amino Acid Analysis by Precolumn Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-I-Alanine Amide (Marfey’s Reagent)
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    Chapter 108 Amino Acid Analysis of Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection
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    Chapter 109 Validation of Amino Acid Analysis Methods
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    Chapter 110 Molecular Weight Estimation for Native Proteins Using High-Performance Size-Exclusion Chromatography
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    Chapter 111 Detection of Disulfide-Linked Peptides by HPLC
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    Chapter 112 Detection of Disulfide-Linked Peptides by Mass Spectrometry
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    Chapter 113 Diagonal Electrophoresis for Detecting Disulfide Bridges
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    Chapter 114 Estimation of Disulfide Bonds Using Ellman’s Reagent
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    Chapter 115 Quantitation of Cysteine Residues and Disulfide Bonds by Electrophoresis
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    Chapter 116 N-Terminal Sequencing of N-Terminally Modified Proteins
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    Chapter 117 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid
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    Chapter 118 Detection and Characterization of Protein Mutations by Mass Spectrometry
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    Chapter 119 Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry
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    Chapter 120 Protein Identification by Peptide Mass Fingerprinting using MALDI-TOF Mass Spectrometry
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    Chapter 121 Protein Ladder Sequencing
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    Chapter 122 Sequence Analysis with WinGene/WinPep
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    Chapter 123 HPLC and Mass Spectrometry of Integral Membrane Proteins
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    Chapter 124 Enrichment of Serum Peptides and Analysis by MALDI-TOF Mass Spectrometry
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    Chapter 125 Computational Methods for Analysis of MALDI-TOF Spectra to Discover Peptide Serum Biomarkers
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    Chapter 126 Simple Tools for Complex N-Glycan Analysis
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    Chapter 127 A Lectin-Binding Assay for the Rapid Characterization of the Glycosylation of Purified Glycoproteins
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    Chapter 128 Chemical Methods of Analysis of Glycoproteins
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    Chapter 129 Monosaccharide Analysis by HPAEC
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    Chapter 130 Monosaccharide Analysis by Gas Chromatography (GC)
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    Chapter 131 Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methylation Analysis
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    Chapter 132 Sialic Acid Analysis by HPAEC-PAD
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    Chapter 133 Chemical Release of O -Linked Oligosaccharide Chains
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    Chapter 134 O -Linked Oligosaccharide Profiling by HPLC
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    Chapter 135 O -Linked Oligosaccharide Profiling by HPAEC-PAD
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    Chapter 136 Release of N -Linked Oligosaccharide Chains by Hydrazinolysis
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    Chapter 137 Enzymatic Release of O -and N -Linked Oligosaccharide Chains
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    Chapter 138 N -Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC)
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    Chapter 139 N -Linked Oligosaccharide Profiling by HPAEC-PAD
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    Chapter 140 HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the CarboPac MA1 Column
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    Chapter 141 Microassay Analyses of Protein Glycosylation
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    Chapter 142 Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins
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    Chapter 143 HPLC Analysis of Fluorescently Labeled Glycans
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    Chapter 144 Glycoprofiling Purified Glycoproteins Using Surface Plasmon Resonance
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    Chapter 145 Sequencing Heparan Sulfate Saccharides
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    Chapter 146 The Protein Protocols Handbook
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    Chapter 147 Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectins
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    Chapter 148 In-Gel Enzymatic Release of N -Glycans
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    Chapter 149 Analysis of N -Linked Glycans by Mass Spectrometry
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    Chapter 150 MS Analysis of Protein Glycosylation
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    Chapter 151 Mapping protein N-Glycosylation by COFRADIC
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    Chapter 152 Mass Spectrometric Analysis of O -Linked Glycans Released Directly from Glycoproteins in Gels Using β-Elimination
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    Chapter 153 Glycopeptide Analysis Using LC/MS and LC/MS n Site-Specific Glycosylation Analysis of a Glycoprotein
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    Chapter 154 Identification of Vitamin K-Dependent Proteins Using a Gla-Specific Monoclonal Antibody
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    Chapter 155 The Identification of Protein S-Nitrosocysteine
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    Chapter 156 Detection of Nitrotyrosine-Containing Proteins
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    Chapter 157 Mass Spectrometric Determination of Protein Ubiquitination
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    Chapter 158 Detection of Sumoylated Proteins
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    Chapter 159 Efficient Enrichment of Intact Phosphorylated Proteins by Modified Immobilized Metal-Affinity Chromatography
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    Chapter 160 Analyzing Protein Phosphorylation
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    Chapter 161 Mass Spectrometric Analysis of Protein Phosphorylation
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    Chapter 162 Protein Microarrays for Phosphorylation Studies
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    Chapter 163 Two-Dimensional Phosphopeptide Mapping
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    Chapter 164 Identification of Proteins Modified by Protein (D-Aspartyl/L-Isoaspartyl) Carboxyl Methyltransferase
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    Chapter 165 Analysis of Tyrosine- O -Sulfation
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    Chapter 166 Analysis of Protein Palmitoylation by Metabolic Radiolabeling Methods
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    Chapter 167 Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins
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    Chapter 168 The Metabolic Labeling and Analysis of Isoprenylated Proteins
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    Chapter 169 Antibody Production
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    Chapter 170 Production of Antibodies Using Proteins in Gel Bands
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    Chapter 171 Raising Highly Specific Polyclonal Antibodies Using Biocompatible Support-Bound Antigens
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    Chapter 172 Production of Antisera Using Peptide Conjugates
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    Chapter 173 Small-Molecule—Protein Conjugation Procedures
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    Chapter 174 The Chloramine T Method for Radiolabeling Protein
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    Chapter 175 The Lactoperoxidase Method for Radiolabeling Protein
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    Chapter 176 The Bolton and Hunter Method for Radiolabeling Protein
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    Chapter 177 Preparation of 125 I-Labeled Peptides and Proteins with High Specific Activity Using IODO-GEN
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    Chapter 178 Purification and Assessment of Quality of Radioiodinated Protein
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    Chapter 179 Purification of IgG by Precipitation with Sodium Sulfate or Ammonium Sulfate
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    Chapter 180 Purification of IgG Using Caprylic Acid
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    Chapter 181 Purification of IgG Using DEAE-Sepharose Chromatography
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    Chapter 182 Purification of IgG Using Ion-Exchange HPLC
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    Chapter 183 Purification of IgG by Precipitation with Polyethylene Glycol (PEG)
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    Chapter 184 Purification of IgG Using Protein A or Protein G
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    Chapter 185 Analysis and Purification of IgG Using Size-Exclusion High Performance Liquid Chromatography (SE-HPLC)
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    Chapter 186 Purification of IgG Using Affinity Chromatography on Antigen-Ligand Columns
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    Chapter 187 Purification of IgG Using Thiophilic Chromatography
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    Chapter 188 Analysis of IgG Fractions by Electrophoresis
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    Chapter 189 Purification of Immunoglobulin Y (IgY) from Chicken Eggs
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    Chapter 190 Affinity Purification of Immunoglobulins Using Protein A Mimetic (PAM)
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    Chapter 191 Detection of Serological Cross-Reactions by Western Cross-Blotting
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    Chapter 192 Enzymatic Digestion of Monoclonal Antibodies
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    Chapter 193 How to Make Bispecific Antibodies
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    Chapter 194 Antigen Measurement Using ELISA
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    Chapter 195 Enhanced Chemiluminescence Immunoassay
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    Chapter 196 Immunoprecipitation and Blotting
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    Chapter 197 Determination of Epitopes by Mass Spectrometry
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    Chapter 198 Immunogen Preparation and Immunization Procedures for Rats and Mice
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    Chapter 199 Making Hybridomas
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    Chapter 200 Growing Hybridomas
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    Chapter 201 Mouse Hybridomas as an Entryway to Monoclonal Antibody Design and Production
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    Chapter 202 Potential Pitfalls in Monoclonal Antibody Development and Applications
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    Chapter 203 Recombinant Antibody Expression and Purification
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    Chapter 204 Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay
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    Chapter 205 Screening Hybridoma Culture Supernatants Using ELISA
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    Chapter 206 Growth and Purification of Murine Monoclonal Antibodies
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    Chapter 207 Affinity Purification Techniques for Monoclonal Antibodies
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    Chapter 208 A Rapid Method for Generating Large Numbers of High-Affinity Monoclonal Antibodies from a Single Mouse
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Title
The Protein Protocols Handbook
Published by
Humana Press, June 2010
DOI 10.1007/978-1-59745-198-7
ISBNs
978-1-60327-474-6, 978-1-59745-198-7
Editors

Walker, John M.

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Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 35%
Student > Ph. D. Student 3 13%
Student > Bachelor 2 9%
Student > Doctoral Student 1 4%
Lecturer 1 4%
Other 2 9%
Unknown 6 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 35%
Agricultural and Biological Sciences 3 13%
Immunology and Microbiology 2 9%
Pharmacology, Toxicology and Pharmaceutical Science 2 9%
Chemical Engineering 1 4%
Other 2 9%
Unknown 5 22%