| Title |
Stimulation of aquaporin-5 and transepithelial water permeability in human airway epithelium by hyperosmotic stress
|
|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, October 2006
|
| DOI | 10.1007/s00424-006-0157-3 |
| Pubmed ID | |
| Authors |
Peter Steen Pedersen, Thomas Hartig Braunstein, Anders Jørgensen, Per Leganger Larsen, Niels-Henrik Holstein-Rathlou, Ole Frederiksen |
| Abstract |
Osmotic water permeability (P(f )) was measured in spheroid-shaped human nasal airway epithelial explants pre-exposed to increasing levels of hyperosmotic stress. The fluid-filled spheroids, derived from nasal polyps, were lined by a single cell layer with the ciliated apical cell membrane facing the outside. The P(f ) was determined from diameter changes of the spheroids in response to changes in bathing medium osmolarity forth and back between 300 and 225 mOsm x l(-1). Continuous diameter measurements also allowed determination of spontaneous fluid absorption. Hyperosmotic pretreatment (increase from 300 up to 600 mOsm x l(-1)) caused a time- and osmolarity-dependent increase (up to approximately 1.5 times) in epithelial P(f ) which was of similar magnitude in cystic fibrosis (CF) and non-CF spheroids. The effect saturated at approximately 450 mOsm x l(-1) and at approximately 24 h. Expression of aquaporin-5 (AQP5), studied by immunofluorescence and confocal microscopy, showed an increase in parallel with the increase in P(f ) following hyperosmotic stress. The AQP5 was localized both in cytoplasmic vesicles and in apical cell membranes. Spontaneous fluid absorption rates were equal in CF and non-CF spheroids and were not significantly influenced by hyperosmotic stress. The results suggest that hyperosmotic stress is an important activator of AQP-5 in human airway epithelium, leading to significantly increased transepithelial water permeability. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 24% |
| Student > Master | 3 | 18% |
| Student > Ph. D. Student | 3 | 18% |
| Student > Doctoral Student | 1 | 6% |
| Professor | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 4 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 41% |
| Biochemistry, Genetics and Molecular Biology | 3 | 18% |
| Chemistry | 2 | 12% |
| Medicine and Dentistry | 1 | 6% |
| Unknown | 4 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 December 2009.
All research outputs
#7,855,444
of 23,815,455 outputs
Outputs from Pflügers Archiv - European Journal of Physiology
#476
of 1,973 outputs
Outputs of similar age
#24,155
of 68,345 outputs
Outputs of similar age from Pflügers Archiv - European Journal of Physiology
#4
of 14 outputs
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