Title |
Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates
|
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Published in |
Biosensors, October 2015
|
DOI | 10.3390/bios5040664 |
Pubmed ID | |
Authors |
Peter Owens, Nigel Phillipson, Jayakumar Perumal, Gerard M. O’Connor, Malini Olivo |
Abstract |
In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration. |
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