| Title |
Isolation of an abundantly expressed sequence from the human X chromosome by differential screening
|
|---|---|
| Published in |
Somatic Cell and Molecular Genetics, January 1988
|
| DOI | 10.1007/bf01535047 |
| Pubmed ID | |
| Authors |
Michael V. Wiles, Caroline M. Alexander, Peter N. Goodfellow |
| Abstract |
A cDNA library was constructed from poly(A)+ RNA derived from MP2H4, a mouse-human somatic cell hybrid, containing as its only human contribution an X-6 translocation chromosome. This library was screened with [32P] c-DNA derived from MP2H4 and counterscreened with a phenotypically similar mouse cell line. From a screen of 4000 recombinants, seven clones were isolated which hybridized more strongly with cDNA derived from the mouse-human hybrid than with the mouse only cell line. Southern blot analysis showed that four of the seven clones originate from the human genome, three of these contain repeat sequences, and one, SCR10, is devoid of repeats. SCR10 identifies a 1-kb mRNA transcribed from the human X chromosome mapping to the region Xq13-q13.3 or Xq21.3-q22 and is an abundantly and ubiquitously expressed gene. A near, or full-length clone of SCR10, SCAR, was isolated and sequenced; the conceptional translation of this sequence encodes a basic protein of 27.5 kd. Sequences homologous to SCAR were detected in primates, rodents, avians, and Xenopus. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 4 | 67% |
| Student > Bachelor | 1 | 17% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 83% |
| Unknown | 1 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2017.
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#7,454,298
of 22,789,076 outputs
Outputs from Somatic Cell and Molecular Genetics
#63
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#9,472
of 49,696 outputs
Outputs of similar age from Somatic Cell and Molecular Genetics
#1
of 3 outputs
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