| Title |
Gef10—the third member of a Rho-specific guanine nucleotide exchange factor subfamily with unusual protein architecture
|
|---|---|
| Published in |
Naunyn-Schmiedeberg's Archives of Pharmacology, July 2006
|
| DOI | 10.1007/s00210-006-0083-0 |
| Pubmed ID | |
| Authors |
M. Mohl, S. Winkler, T. Wieland, S. Lutz |
| Abstract |
According to cDNA sequence homologies, Gef10 is related to the Rho-specific guanine nucleotide exchange factors GrinchGEF and p164-RhoGEF. Like these GEFs, Gef10 exhibits only weak homology to known pleckstrin homology domains, but contains a putative WD40-like domain. As detected by RT-PCR, Gef10 is transcribed in at least two splice variants in different human tissues. Although the Gef10 sequence contains two putative transmembrane segments, recombinantly expressed Gef10 displays a cytosolic localisation. As detected by guanine nucleotide exchange activity assay, precipitation assay of GTP-bound Rho proteins and serum response element dependent gene transcription Gef10 activates RhoA-C, but not Rac1 or Cdc42. In the reporter gene assay, Gef10 preferentially activated RhoB. When expressed in NIH3T3 cells, Gef10 induced actin stress fibre, but not lamellipodia or filopodia formation. We conclude that Gef10 is the third member of a Rho-specific GEF family with unusual protein architecture. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 31 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 25% |
| Student > Master | 8 | 25% |
| Student > Ph. D. Student | 3 | 9% |
| Professor | 2 | 6% |
| Professor > Associate Professor | 2 | 6% |
| Other | 5 | 16% |
| Unknown | 4 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 53% |
| Medicine and Dentistry | 3 | 9% |
| Biochemistry, Genetics and Molecular Biology | 3 | 9% |
| Unspecified | 1 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 4 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#7,454,298
of 22,789,076 outputs
Outputs from Naunyn-Schmiedeberg's Archives of Pharmacology
#347
of 1,724 outputs
Outputs of similar age
#22,751
of 65,444 outputs
Outputs of similar age from Naunyn-Schmiedeberg's Archives of Pharmacology
#2
of 5 outputs
Altmetric has tracked 22,789,076 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,724 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
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We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.