| Title |
Transgenic modification of gai or rgl1 causes dwarfing and alters gibberellins, root growth, and metabolite profiles in Populus
|
|---|---|
| Published in |
Planta, January 2006
|
| DOI | 10.1007/s00425-005-0213-9 |
| Pubmed ID | |
| Authors |
Victor Busov, Richard Meilan, David W. Pearce, Stewart B. Rood, Caiping Ma, Timothy J. Tschaplinski, Steven H. Strauss |
| Abstract |
In Arabidopsis and other plants, gibberellin (GA)-regulated responses are mediated by proteins including GAI, RGA and RGL1-3 that contain a functional DELLA domain. Through transgenic modification, we found that DELLA-less versions of GAI (gai) and RGL1 (rgl1) in a Populus tree have profound, dominant effects on phenotype, producing pleiotropic changes in morphology and metabolic profiles. Shoots were dwarfed, likely via constitutive repression of GA-induced elongation, whereas root growth was promoted two- to threefold in vitro. Applied GA(3 )inhibited adventitious root production in wild-type poplar, but gai/rgl1 poplars were unaffected by the inhibition. The concentrations of bioactive GA(1) and GA(4) in leaves of gai- and rgl1-expressing plants increased 12- to 64-fold, while the C(19) precursors of GA(1) (GA(53), GA(44) and GA(19)) decreased three- to ninefold, consistent with feedback regulation of GA 20-oxidase in the transgenic plants. The transgenic modifications elicited significant metabolic changes. In roots, metabolic profiling suggested increased respiration as a possible mechanism of the increased root growth. In leaves, we found metabolite changes suggesting reduced carbon flux through the lignin biosynthetic pathway and a shift towards allocation of secondary storage and defense metabolites, including various phenols, phenolic glucosides, and phenolic acid conjugates. |
Mendeley readers
The data shown below were compiled from readership statistics for 109 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 3 | 3% |
| Spain | 2 | 2% |
| Norway | 1 | <1% |
| Israel | 1 | <1% |
| United Kingdom | 1 | <1% |
| France | 1 | <1% |
| Japan | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 98 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 22% |
| Researcher | 16 | 15% |
| Student > Master | 14 | 13% |
| Student > Doctoral Student | 11 | 10% |
| Professor | 8 | 7% |
| Other | 24 | 22% |
| Unknown | 12 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 72 | 66% |
| Biochemistry, Genetics and Molecular Biology | 11 | 10% |
| Medicine and Dentistry | 4 | 4% |
| Environmental Science | 3 | 3% |
| Engineering | 2 | 2% |
| Other | 1 | <1% |
| Unknown | 16 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 January 2023.
All research outputs
#3,920,371
of 24,652,720 outputs
Outputs from Planta
#131
of 2,885 outputs
Outputs of similar age
#12,572
of 162,025 outputs
Outputs of similar age from Planta
#1
of 21 outputs
Altmetric has tracked 24,652,720 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,885 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 162,025 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.