| Title |
Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix
|
|---|---|
| Published in |
Lasers in Medical Science, November 2005
|
| DOI | 10.1007/s10103-005-0355-9 |
| Pubmed ID | |
| Authors |
Liat Abramovitch-Gottlib, Talia Gross, Doron Naveh, Shimona Geresh, Salman Rosenwaks, Ilana Bar, Razi Vago |
| Abstract |
Mesenchymal stem cells (MSCs) seeded on three-dimensional (3D) coralline (Porites lutea) biomatrices were irradiated with low-level laser irradiation (LLLI). The consequent phenotype modulation and development of MSCs towards ossified tissue was studied in this combined 3D biomatrix/LLLI system and in a control group, which was similarly grown, but was not treated by LLLI. The irradiated and non irradiated MSC were tested at 1-7, 10, 14, 21, 28 days of culturing via analysis of cellular distribution on matrices (trypan blue), calcium incorporation to newly formed tissue (alizarin red), bone nodule formation (von Kossa), fat aggregates formation (oil red O), alkaline phosphatase (ALP) activity, scanning electron microscopy (SEM) and electron dispersive spectrometry (EDS). The results obtained from the irradiated samples showed enhanced tissue formation, appearance of phosphorous peaks and calcium and phosphate incorporation to newly formed tissue. Moreover, in irradiated samples ALP activity was significantly enhanced in early stages and notably reduced in late stages of culturing. These findings of cell and tissue parameters up to 28 days of culture revealed higher ossification levels in irradiated samples compared with the control group. We suggest that both the surface properties of the 3D crystalline biomatrices and the LLLI have biostimulatory effects on the conversion of MSCs into bone-forming cells and on the induction of ex-vivo ossification. |
Mendeley readers
The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 1% |
| Unknown | 78 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 15% |
| Student > Bachelor | 8 | 10% |
| Researcher | 7 | 9% |
| Student > Master | 6 | 8% |
| Professor > Associate Professor | 5 | 6% |
| Other | 21 | 27% |
| Unknown | 20 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 23 | 29% |
| Engineering | 8 | 10% |
| Agricultural and Biological Sciences | 8 | 10% |
| Biochemistry, Genetics and Molecular Biology | 7 | 9% |
| Environmental Science | 2 | 3% |
| Other | 8 | 10% |
| Unknown | 23 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2015.
All research outputs
#7,454,298
of 22,789,076 outputs
Outputs from Lasers in Medical Science
#278
of 1,308 outputs
Outputs of similar age
#16,760
of 48,666 outputs
Outputs of similar age from Lasers in Medical Science
#1
of 1 outputs
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