| Title |
The lactate dehydrogenase-elevating virus capsid protein is a nuclear–cytoplasmic protein
|
|---|---|
| Published in |
Archives of Virology, June 2009
|
| DOI | 10.1007/s00705-009-0410-0 |
| Pubmed ID | |
| Authors |
Hakimeh Mohammadi, Shayan Sharif, Raymond R. Rowland, Dongwan Yoo |
| Abstract |
Arteriviruses replicate in the cytoplasm and do not require the nucleus function for virus multiplication in vitro. However, nucleocapsid (N) protein of two arteriviruses, porcine reproductive respiratory syndrome virus and equine arteritis virus, has been observed to localize in the nucleus and nucleolus of virus-infected and N-gene-transfected cells in addition to their normal cytoplasmic distribution. In the present study, the N protein of lactate dehydrogenase-elevating virus (LDV) of mice was examined for nuclear localization. The subcellular localization of LDV-N was determined by tagging N with enhanced green fluorescence protein (EGFP) at the N- and C-terminus. Both N-EGFP and EGFP-N fusion proteins localized to the nucleus and nucleolus of gene-transfected cells. Labeled N also accumulated in the perinuclear region, the site of virus replication. The LDV-N sequence contains a putative 'pat4'-type nuclear localization signal (NLS) consisting of 38-KKKK. To determine its functional significance, a series of deletion constructs of N were generated and individually expressed in cells. The results showed that the 'pat4' NLS was essential for nuclear translocation. In addition, the LDV-N interacted with the importin-alpha and -beta proteins, suggesting that the LDV-N nuclear localization may occur via the importin-mediated nuclear transport pathway. These results provide further evidence for the nuclear localization of N as a common feature within the arteriviruses. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 1 | 8% |
| Unknown | 12 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 31% |
| Professor | 3 | 23% |
| Student > Ph. D. Student | 2 | 15% |
| Student > Doctoral Student | 1 | 8% |
| Other | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 2 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 31% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Immunology and Microbiology | 2 | 15% |
| Agricultural and Biological Sciences | 2 | 15% |
| Unknown | 3 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 September 2020.
All research outputs
#7,454,298
of 22,789,076 outputs
Outputs from Archives of Virology
#916
of 4,144 outputs
Outputs of similar age
#38,119
of 112,549 outputs
Outputs of similar age from Archives of Virology
#6
of 14 outputs
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